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DBBM shows no signs of resorption under inflammatory conditions. An experimental study in the mouse calvaria.

ABSTRACT: OBJECTIVES:Deproteinized bovine bone mineral (DBBM) is not resorbable. However, the behavior of DBBM under inflammatory conditions remains unclear. Aim of the study was therefore to evaluate the resorption of DBBM under local inflammatory conditions in vivo using the calvarial osteolysis model. METHODS:In thirty adult BALB/c mice, DBBM was implanted into the space between the elevated soft tissue and the calvarial bone. Inflammation was induced either by lipopolysaccharides (LPS) injection or by polyethylene particles (Ceridust) mixed with DBBM. Three modalities were randomly applied (n = 10 each): (a) DBBM alone (control), (b) DBBM + LPS, and (c) DBBM + polyethylene particles (Ceridust). Mice were euthanized on day fourteen, and each calvarium was subjected to histological and µCT analysis. Primary outcome was the size distribution of the DBBM particles. Secondary outcome was the surface erosion of the calvarial bone. RESULTS:Histological and µCT analysis revealed that the size distribution and the volume of DBBM particles in the augmented site were similar between DBBM alone and the combinations with LPS or polyethylene particles. Moreover, histological evaluation showed no signs of erosions of DBBM particles under inflammatory conditions. µCT analysis and histology further revealed that LPS and the polyethylene particles, but not the DBBM alone, caused severe erosions of the calvarial bone as indicated by large voids representing the massive compensatory new immature woven bone formation on the endosteal surface. CONCLUSIONS:Local calvarial bone but not the DBBM particles undergo severe resorption and subsequent new bone formation under inflammatory conditions in a mouse model.

SUBMITTER: Kuchler U

PROVIDER: S-EPMC7003744 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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DBBM shows no signs of resorption under inflammatory conditions. An experimental study in the mouse calvaria.

Kuchler Ulrike U Dos Santos Gabriel Mulinari GM Heimel Patrick P Stähli Alexandra A Strauss Franz Josef FJ Tangl Stefan S Gruber Reinhard R

Clinical oral implants research 20190930 1

<h4>Objectives</h4>Deproteinized bovine bone mineral (DBBM) is not resorbable. However, the behavior of DBBM under inflammatory conditions remains unclear. Aim of the study was therefore to evaluate the resorption of DBBM under local inflammatory conditions in vivo using the calvarial osteolysis model.<h4>Methods</h4>In thirty adult BALB/c mice, DBBM was implanted into the space between the elevated soft tissue and the calvarial bone. Inflammation was induced either by lipopolysaccharides (LPS) ...[more]

PMID: 31529644

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Project description:BackgroundThe application of orthodontic forces causes root resorption of variable severity with potentially severe clinical ramifications.ObjectiveTo systematically review reports on the pathophysiological mechanisms of orthodontically induced inflammatory root resorption (OIIRR) and the associated risk factors based on in vitro, experimental, and in vivo studies.Search methodsWe undertook an electronic search of four databases and a separate hand-search.Selection criteriaStudies reporting on the effect of orthodontic forces with/without the addition of potential risk factors on OIIRR, including (1) gene expression in in-vitro studies, the incidence root resorption in (2) animal studies, and (3) human studies.Data collection and analysisPotential hits underwent a two-step selection, data extraction, quality assessment, and systematic appraisal performed by duplicate examiners.ResultsOne hundred and eighteen articles met the eligibility criteria. Studies varied considerably in methodology, reporting of results, and variable risk of bias judgements.In summary, the variable evidence identified supports the notion that the application of orthodontic forces leads to (1) characteristic alterations of molecular expression profiles in vitro, (2) an increased rate of OIIRR in animal models, as well as (3) in human studies. Importantly, the additional presence of risk factors such as malocclusion, previous trauma, and medications like corticosteroids increased the severity of OIIRR, whilst other factors decreased its severity, including oral contraceptives, baicalin, and high caffeine.ConclusionsBased on the systematically reviewed evidence, OIIRR seems to be an inevitable consequence of the application of orthodontic forces-with different risk factors modifying its severity. Our review has identified several molecular mechanisms that can help explain this link between orthodontic forces and OIIRR. Nevertheless, it must be noted that the available eligible literature was in part significantly confounded by bias and was characterized by substantial methodological heterogeneity, suggesting that the results of this systematic review should be interpreted with caution.RegistrationPROSPERO (CRD42021243431).

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DBBM shows no signs of resorption under inflammatory conditions. An experimental study in the mouse calvaria.

Publications

DBBM shows no signs of resorption under inflammatory conditions. An experimental study in the mouse calvaria.

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