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Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy.

ABSTRACT: Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.

SUBMITTER: de Medeiros G

PROVIDER: S-EPMC7005178 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy.

de Medeiros Gustavo G Kromm Dimitri D Balazs Balint B Norlin Nils N Günther Stefan S Izquierdo Emiliano E Ronchi Paolo P Komoto Shinya S Krzic Uros U Schwab Yannick Y Peri Francesca F de Renzis Stefano S Leptin Maria M Rauzi Matteo M Hufnagel Lars L

Scientific reports 20200206 1

Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses ...[more]

PMID: 32029815

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