Ontology highlight
ABSTRACT:
SUBMITTER: Engelmann S
PROVIDER: S-EPMC7005293 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Scientific reports 20200206 1
We developed a time-efficient semi-automated axon quantification method using freeware in human cranial nerve sections stained with paraphenylenediamine (PPD). It was used to analyze a total of 1238 facial and masseteric nerve biopsies. The technique was validated by comparing manual and semi-automated quantification of 129 (10.4%) randomly selected biopsies. The software-based method demonstrated a sensitivity of 94% and a specificity of 87%. Semi-automatic axon counting was significantly faste ...[more]