Unknown

Dataset Information

0

M2 macrophages predict worse long-term outcomes in human acute tubular necrosis.


ABSTRACT: Although macrophages are important players in the injury/repair processes in animal models of acute kidney injury (AKI), their roles in human AKI remains uncertain owing to a paucity of human biopsy studies. We investigated the role of macrophages in 72 cases of biopsy-proven acute tubular necrosis (ATN) and six cases of healthy kidney. Macrophages were identified by CD68 and CD163 immunohistochemistry and analyzed for their effect on renal outcomes. CD163+ M2 macrophages outnumbered CD68+ cells in the healthy kidneys, suggesting that CD163+ macrophages are resident macrophages. The infiltration of both subtypes of macrophages increased significantly in ATN. The density of the CD68+ macrophages was significantly higher in advanced-stage AKI, whereas CD163+ M2 macrophages was not. Eighty percent of patients exhibited renal functional recovery during follow-up. Older age and a higher density of CD163+ macrophages predicted non-recovery, whereas the AKI stage, tubular injury score, and density of CD68+ cells did not. The density of CD163+ M2 macrophages was an independent predictor of low eGFR at 3 months in advanced-stage AKI. This is the first human study demonstrating the possible role of macrophages in the injury and repair phases of AKI.

SUBMITTER: Kim MG 

PROVIDER: S-EPMC7005727 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

M2 macrophages predict worse long-term outcomes in human acute tubular necrosis.

Kim Myung-Gyu MG   Lim Kijoon K   Lee Yoo Jin YJ   Yang Jihyun J   Oh Se Won SW   Cho Won Yong WY   Jo Sang-Kyung SK  

Scientific reports 20200207 1


Although macrophages are important players in the injury/repair processes in animal models of acute kidney injury (AKI), their roles in human AKI remains uncertain owing to a paucity of human biopsy studies. We investigated the role of macrophages in 72 cases of biopsy-proven acute tubular necrosis (ATN) and six cases of healthy kidney. Macrophages were identified by CD68 and CD163 immunohistochemistry and analyzed for their effect on renal outcomes. CD163+ M2 macrophages outnumbered CD68+ cells  ...[more]

Similar Datasets

2016-01-15 | E-GEOD-76882 | biostudies-arrayexpress
2016-01-15 | GSE76882 | GEO
| S-EPMC5501990 | biostudies-literature
| S-EPMC6014357 | biostudies-literature
| S-EPMC9475290 | biostudies-literature
| S-EPMC7737431 | biostudies-literature
| S-EPMC4145550 | biostudies-literature
2024-09-09 | PXD016051 | Pride
2020-12-21 | PXD016379 | Pride
| S-EPMC10436571 | biostudies-literature