Ontology highlight
ABSTRACT:
SUBMITTER: Teets EM
PROVIDER: S-EPMC7005852 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Teets Emily M EM Gregory Charles C Shaffer Jami J Blachly James S JS Blaser Bradley W BW
Scientific reports 20200207 1
Hematopoietic stem cells (HSCs) are functionally and genetically diverse and this diversity decreases with age and disease. Numerous systems have been developed to quantify HSC diversity by genetic barcoding, but no framework has been established to empirically validate barcode sequences. Here we have developed an analytical framework, Selection of informative Amplicon Barcodes from Experimental Replicates (SABER), that identifies barcodes that are unique among a large set of experimental replic ...[more]