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Core-Shell NaHoF₄@TiO₂ NPs: A Labeling Method to Trace Engineered Nanomaterials of Ubiquitous Elements in the Environment.

ABSTRACT: Understanding the fate and behavior of nanoparticles (NPs) in the natural environment is important to assess their potential risk. Single particle inductively coupled plasma mass spectrometry (spICP-MS) allows for the detection of NPs at extremely low concentrations, but the high natural background of the constituents of many of the most widely utilized nanoscale materials makes accurate quantification of engineered particles challenging. Chemical doping, with a less naturally abundant element, is one approach to address this; however, certain materials with high natural abundance, such as TiO₂ NPs, are notoriously difficult to label and differentiate from natural NPs. Using the low abundance rare earth element Ho as a marker, Ho-bearing core -TiO₂ shell (NaHoF₄@TiO₂) NPs were designed to enable the quantification of engineered TiO₂ NPs in real environmental samples. The NaHoF₄@TiO₂ NPs were synthesized on a large scale (gram), at relatively low temperatures, using a sacrificial Al(OH)₃ template that confines the hydrolysis of TiF₄ within the space surrounding the NaHoF₄ NPs. The resulting NPs consist of a 60 nm NaHoF₄ core and a 5 nm anatase TiO₂ shell, as determined by TEM, STEM-EDX mapping, and spICP-MS. The NPs exhibit excellent detectability by spICP-MS at extremely low concentrations (down to 1 × 10^-3 ng/L) even in complex natural environments with high Ti background.

SUBMITTER: Cui X

PROVIDER: S-EPMC7006996 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:BackgroundTiO2 nanomaterials (NMs) are present in a variety of food and personal hygiene products, and consumers are exposed daily to these NMs through oral exposition. While the bulk of ingested TiO2 NMs are eliminated rapidly in stool, a fraction is able to cross the intestinal epithelial barrier and enter systemic circulation from where NMs can be distributed to tissues, primarily liver and spleen. Daily exposure to TiO2 NMs, in combination with a slow rate of elimination from tissues, results in their accumulation within different tissues. Considerable evidence suggests that following oral exposure to TiO2 NMs, the presence of NMs in tissues is associated with a number of adverse effects, both in intestine and liver. Although numerous studies have been performed in vitro investigating the acute effects of TiO2 NMs in intestinal and hepatic cell models, considerably less is known about the effect of repeated exposure on these models. In this study, we investigated the cytotoxic effects of repeated exposure of relevant models of intestine and liver to two TiO2 NMs differing in hydrophobicity for 24 h, 1 week and 2 weeks at concentrations ranging from 0.3 to 80 µg/cm2. To study the persistence of these two NMs in cells, we included a 1-week recovery period following 24 h and 1-week treatments. Cellular uptake by TEM and ToF-SIMS analyses, as well as the viability and pro-inflammatory response were evaluated. Changes in the membrane composition in Caco-2 and HepaRG cells treated with TiO2 NMs for up to 2 weeks were also studied.ResultsDespite the uptake of NM-103 and NM-104 in cells, no significant cytotoxic effects were observed in either Caco-2 or HepaRG cells treated for up to 2 weeks at NM concentrations up to 80 µg/cm2. In addition, no significant effects on IL-8 secretion were observed. However, significant changes in membrane composition were observed in both cell lines. Interestingly, while most of these phospholipid modifications were reversed following a 1-week recovery, others were not affected by the recovery period.ConclusionThese findings indicate that although no clear effects on cytotoxicity were observed following repeated exposure of differentiated Caco-2 and HepaRG cells to TiO2 NMs, subtle effects on membrane composition could induce potential adverse effects in the long-term.

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Core-Shell NaHoF₄@TiO₂ NPs: A Labeling Method to Trace Engineered Nanomaterials of Ubiquitous Elements in the Environment.

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Core-Shell NaHoF4@TiO2 NPs: A Labeling Method to Trace Engineered Nanomaterials of Ubiquitous Elements in the Environment.

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Core-Shell NaHoF₄@TiO₂ NPs: A Labeling Method to Trace Engineered Nanomaterials of Ubiquitous Elements in the Environment.