Ontology highlight
ABSTRACT:
SUBMITTER: Ouldali H
PROVIDER: S-EPMC7008938 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Nature biotechnology 20191216 2
Efforts to sequence single protein molecules in nanopores<sup>1-5</sup> have been hampered by the lack of techniques with sufficient sensitivity to discern the subtle molecular differences among all twenty amino acids. Here we report ionic current detection of all twenty proteinogenic amino acids in an aerolysin nanopore with the help of a short polycationic carrier. Application of molecular dynamics simulations revealed that the aerolysin nanopore has a built-in single-molecule trap that fully ...[more]