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Ralstonia solanacearum Type III Effectors with Predicted Nuclear Localization Signal Localize to Various Cell Compartments and Modulate Immune Responses in Nicotiana spp.


ABSTRACT: Ralstonia solanacearum (Rso) is a causal agent of bacterial wilt in Solanaceae crops worldwide including Republic of Korea. Rso virulence predominantly relies on type III secreted effectors (T3Es). However, only a handful of Rso T3Es have been characterized. In this study, we investigated subcellular localization of and manipulation of plant immunity by 8 Rso T3Es predicted to harbor a nuclear localization signal (NLS). While 2 of these T3Es elicited cell death in both Nicotiana benthamiana and N. tabacum, only one was dependent on suppressor of G2 allele of skp1 (SGT1), a molecular chaperone of nucleotide-binding and leucine-rich repeat immune receptors. We also identified T3Es that differentially regulate flg22-induced reactive oxygen species production and gene expression. Interestingly, several of the NLS-containing T3Es translationally fused with yellow fluorescent protein accumulated in subcellular compartments other than the cell nucleus. Our findings bring new clues to decipher Rso T3E function in planta.

SUBMITTER: Jeon H 

PROVIDER: S-EPMC7012579 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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<i>Ralstonia solanacearum</i> Type III Effectors with Predicted Nuclear Localization Signal Localize to Various Cell Compartments and Modulate Immune Responses in <i>Nicotiana</i> spp.

Jeon Hyelim H   Kim Wanhui W   Kim Boyoung B   Lee Sookyeong S   Jayaraman Jay J   Jung Gayoung G   Choi Sera S   Sohn Kee Hoon KH   Segonzac Cécile C  

The plant pathology journal 20200201 1


<i>Ralstonia solanacearum</i> (<i>Rso</i>) is a causal agent of bacterial wilt in <i>Solanaceae</i> crops worldwide including Republic of Korea. <i>Rso</i> virulence predominantly relies on type III secreted effectors (T3Es). However, only a handful of <i>Rso</i> T3Es have been characterized. In this study, we investigated subcellular localization of and manipulation of plant immunity by 8 <i>Rso</i> T3Es predicted to harbor a nuclear localization signal (NLS). While 2 of these T3Es elicited cel  ...[more]

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