GABA_A Receptor-Mediated Currents and Hormone mRNAs in Cells Expressing More Than One Hormone Transcript in Intact Human Pancreatic Islets.

ABSTRACT: In pancreatic islets, the major cell-types are α, β and δ cells. The γ-aminobutyric acid (GABA) signalling system is expressed in human pancreatic islets. In single hormone transcript-expressing cells, we have previously characterized the functional properties of islet GABA_A receptors (iGABA_ARs). Here, we extended these studies to islet cells expressing mRNAs for more than one hormone and sought for correlation between iGABA_AR activity level and relative mRNA expression ratio. The single-cell RT-PCR in combination with the patch-clamp current recordings was used to examine functional properties of iGABA_ARs in the multiple hormone mRNA-expressing cells. We detected cells expressing double (α/β, α/δ, β/δ cell-types) and triple (α/β/δ cell-type) hor

SUBMITTER: Korol SV 

PROVIDER: S-EPMC7013858 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature