Characterization of HIV-1 diversity in various compartments at the time of primary infection by ultradeep sequencing.

ABSTRACT: We used next-generation sequencing to evaluate the quantity and genetic diversity of the HIV envelope gene in various compartments in eight patients with acute infection. Plasma (PL) and seminal fluid (SF) were available for all patients, whole blood (WB) for seven, non-spermatozoid cells (NSC) for four, and saliva (SAL) for three. Median HIV-1 RNA was 6.2 log₁₀ copies/mL [IQR: 5.5-6.95] in PL, 4.9 log₁₀ copies/mL [IQR: 4.25-5.29] in SF, and 4.9 log₁₀ copies/mL [IQR: 4.46-5.09] in SAL. Median HIV-1 DNA was 4.1 log₁₀ copies/10⁶ PBMCs [IQR: 3.15-4.15] in WB and 2.6 log₁₀ copies /10⁶ Cells [IQR: 2.23-2.75] in NSC. The median overall diversity per patient varied from 0.0005 to 0.0232, suggesting very low diversity, co

SUBMITTER: Gaube G 

PROVIDER: S-EPMC7016127 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature