Decursin and Decursinol Angelate Suppress Adipogenesis through Activation of ?-catenin Signaling Pathway in Human Visceral Adipose-Derived Stem Cells.

ABSTRACT: Visceral adiposity is closely associated with metabolic disorders and cardiovascular diseases. Angelica gigas Nakai (AGN) has been reported to possess anti-obesity effects and higher amounts of coumarin compounds are present in AGN. However, the active compounds suppressing adipogenesis in AGN and mechanisms of action have not been investigated in adipose-derived stem cells (ASCs) isolated from visceral adipose tissue (VAT). Among four coumarin compounds of AGN, decursin (D) and decursinol angelate (DA) significantly inhibited adipocyte differentiation from ASCs. D and DA downregulated CCAAT/enhancer binding protein ? (C/EBP?), peroxisome proliferator-activated receptor ? (PPAR?), adipocyte fatty acid binding protein (aP2), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) at both mRNA and protein levels. Next, treatment with adipogenic differentiation medium (ADM) on ASCs downregulated ?-catenin expression at protein level, while addition of D and DA could restore protein expression and nuclear translocation of ?-catenin suppressed by ADM. D and DA treatment on ADM treated ASCs increased inhibitory phosphorylation of Glycogen synthase kinase (GSK)-3?, thereby preventing ?-catenin from degradation. Additionally, si-?-catenin transfection significantly upregulated protein expression of C/EBP? and PPAR?, alleviating the anti-adipogenic effect of D and DA on ADM treated ASCs. Overall, D and DA, active compounds from AGN, suppressed adipogenesis through activation of ?-catenin signaling pathway in ASCs derived from human VAT, possibly using as natural anti-visceral adiposity agents.

SUBMITTER: Park IS 

PROVIDER: S-EPMC7020042 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature