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Fast Track to Discover Novel Promoters in Rice.


ABSTRACT: Promoters are key components for the application of biotechnological techniques in crop plants. Reporter genes such as GUS or GFP have been used to test the activity of promoters for diverse applications. A huge number of T-DNAs carrying promoterless GUS near their right borders have been inserted into the rice genome, and 105,739 flanking sequence tags from rice lines with this T-DNA insertion have been identified, establishing potential promoter trap lines for 20,899 out of 55,986 genes in the rice genome. Anatomical meta-expression data and information on abiotic stress related to these promoter trap lines enable us to quickly identify new promoters associated with various expression patterns. In the present report, we introduce a strategy to identify new promoters in a very short period of time using a combination of meta-expression analysis and promoter trap lines.

SUBMITTER: Yoo YH 

PROVIDER: S-EPMC7020180 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Fast Track to Discover Novel Promoters in Rice.

Yoo Yo-Han YH   Kim Yu-Jin YJ   Moon Sunok S   Gho Yun-Shil YS   Hong Woo-Jong WJ   Kim Eui-Jung EJ   Jiang Xu X   Jung Ki-Hong KH  

Plants (Basel, Switzerland) 20200118 1


Promoters are key components for the application of biotechnological techniques in crop plants. Reporter genes such as <i>GUS</i> or <i>GFP</i> have been used to test the activity of promoters for diverse applications. A huge number of T-DNAs carrying promoterless <i>GUS</i> near their right borders have been inserted into the rice genome, and 105,739 flanking sequence tags from rice lines with this T-DNA insertion have been identified, establishing potential promoter trap lines for 20,899 out o  ...[more]

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