Dataset Information

Modified QuEChERS Extraction and HPLC-MS/MS for Simultaneous Determination of 155 Pesticide Residues in Rice (Oryza sativa L.).

ABSTRACT: Rice (Oryza sativa L.) is the staple food of more than half of the world's population. The main factors affecting the quality of rice include grain length, texture, stickiness, flavor, and aroma. Pesticides are intended for the protection of plant products from weeds, fungi, or insects. However, pesticides also result in negative effects such as environment disturbances, pest resistance and toxicity to both users and food consumers. The aim of this study was to conduct validation experiments of a method for the determination of multi-pesticides in rice, a model food of other cereals. A quick, easy, cheap, effective, rugged, and safe (QuEChERS) method was used for the extraction of pesticide residues from rice followed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with a triple quadrupole instrument using electrospray ionization. The analytical method has chromatography-tandem according to SANTE/11813/2017. The limit of quantification was 5 ?g/kg. Recoveries for the 155 analyzed pesticides ranged between 77.1% for pirimiphos-ethyl and 111.5% for flutriafol and they were determined at 3 spiking levels. The proposed method was demonstrated to be quick, simple, precise, and accurate and allowed for evaluating the compliance of cereals samples with legislated maximum residue levels of pesticides in the European Union.

SUBMITTER: Melo MG

PROVIDER: S-EPMC7022397 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Modified QuEChERS Extraction and HPLC-MS/MS for Simultaneous Determination of 155 Pesticide Residues in Rice (<i>Oryza sativa</i> L.).

Melo Maria Graça MG Carqueijo Ana A Freitas Andreia A Barbosa Jorge J Silva Ana Sanches AS

Foods (Basel, Switzerland) 20191224 1

Rice (<i>Oryza sativa</i> L.) is the staple food of more than half of the world's population. The main factors affecting the quality of rice include grain length, texture, stickiness, flavor, and aroma. Pesticides are intended for the protection of plant products from weeds, fungi, or insects. However, pesticides also result in negative effects such as environment disturbances, pest resistance and toxicity to both users and food consumers. The aim of this study was to conduct validation experime ...[more]

PMID: 31878165

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Project description:New analytical approaches to the simultaneous identification and quantification of 94 pesticides and 13 polycyclic aromatic hydrocarbons (PAHs) in five representative matrices (pepper, apple, lettuce, wheat, and soil) were developed. The analyses were based on gas chromatography coupled with triple quadrupole tandem mass spectrometry (GC-MS/MS). The procedure was optimized by changing the solvent used during the extraction, from acetonitrile to the acetone: n-hexane mixture at a volume ratio of 1:4 (v/v), as well as the use of a reduced amount of water during the extraction of compounds from cereals. An additional modification was the use of florisil instead of GCB in the sample cleanup step. A full method validation study was performed, at two concentration levels (LOQ and 1000 × LOQ), which showed satisfactory results for all analytes from the PAHs group, with recoveries ranging from 70.7-115.1%, and an average RSD of 3.9%. Linearity was tested in the range of 0.001-1.000 mg/kg and showed coefficients of determination (R2) ≥ 0.99 for all PAHs. Satisfactory recovery and precision parameters (LOQ and 100 × LOQ) were achieved for almost all analytes from the pesticide group in the range of 70.1-119.3% with the mean RSD equal to 5.9%. The observed linearity for all analytes in the concentration range of 0.005-1.44 mg/kg was R2 ≥ 0.99, with the exception of famoxadone, chizalofop-p-ethyl, prothioconazole, spirodiclofen, tefluthrin, and zoxamid. The extended uncertainties were estimated, using a top-down approach of 9.9% (average) and 15.3% (average) for PAHs and pesticides samples, respectively (the coverage factor k = 2, the 95% confidence level). Ultimately, the method was successfully applied to determine pesticide residues in commercial samples of fruit, vegetables and grain, and soil samples for PAHs, which were collected from selected places in the Podkarpacie region. A total of 38 real samples were tested, in which 10 pesticides and 13 PAHs were determined. Proposed changes allow us to shorten the sample preparation time (by 20%) and to reduce the consumption of organic solvents (by 17%). The use of florisil for sample cleanup, instead of GCB, improves the recovery of compounds with flat particles.

Dataset Information

Modified QuEChERS Extraction and HPLC-MS/MS for Simultaneous Determination of 155 Pesticide Residues in Rice (Oryza sativa L.).

Publications

Modified QuEChERS Extraction and HPLC-MS/MS for Simultaneous Determination of 155 Pesticide Residues in Rice (<i>Oryza sativa</i> L.).

Similar Datasets

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