Elucidating the Binding Mechanism of a Novel Silica-Binding Peptide.
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ABSTRACT:
SUBMITTER: Bansal R
PROVIDER: S-EPMC7022404 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Publications
Elucidating the Binding Mechanism of a Novel Silica-Binding Peptide.
Bansal Rachit R Elgundi Zehra Z Care Andrew A C Goodchild Sophia S S Lord Megan M Rodger Alison A Sunna Anwar A
Biomolecules 20191218 1
Linker-protein G (LPG) is a bifunctional fusion protein composed of a solid-binding peptide (SBP, referred as the "linker") with high affinity to silica-based compounds and a <i>Streptococcus</i> protein G (PG), which binds antibodies. The binding mechanisms of LPG to silica-based materials was studied using different biophysical techniques and compared to that of PG without the linker. LPG displayed high binding affinity to a silica surface (<i>K</i><sub>D</sub> = 34.77 ± 11.8 nM), with a verti ...[more]
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