Identification of the NADP⁺ Structural Binding Site and Coenzyme Effect on the Fused G6PD::6PGL Protein from Giardia lamblia.

ABSTRACT: Giardia lambia is a flagellated protozoan parasite that lives in the small intestine and is the causal agent of giardiasis. It has been reported that G. lamblia exhibits glucose-6-phosphate dehydrogenase (G6PD), the first enzyme in the pentose phosphate pathway (PPP). Our group work demonstrated that the g6pd and 6pgl genes are present in the open frame that gives rise to the fused G6PD::6PGL protein; where the G6PD region is similar to the 3D structure of G6PD in Homo sapiens. The objective of the present work was to show the presence of the structural NADP⁺ binding site on the fused G6PD::6PGL protein and evaluate the effect of the NADP⁺ molecule on protein stability using biochemical and computational analysis. A protective effect

SUBMITTER: Morales-Luna L 

PROVIDER: S-EPMC7022596 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature