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A Green, Rapid and Efficient Dual-Sensors for Highly Selective and Sensitive Detection of Cation (Hg2+) and Anion (S2-) Ions Based on CMS/AgNPs Composites.

ABSTRACT: Detection of mercury (Hg2+) and sulfide (S2-), universal and well-known toxic ions, is crucial in monitoring several diseases. How to design and fabricate the high-performance sensor for simultaneously and accurately detecting the Hg2+ and S2- is critical. Herein, we proposed a novel and convenient strategy for optical detection of Hg2+ and S2- by employing a carboxymethyl cellulose sodium/silver nanoparticle (CMS/AgNPs) colloidal solution, in which AgNPs were used as monitor for Hg2+ and S2-, and the CMS was utilized as both the stabilizer and the hydrophilic substrate for AgNPs. Well-identifiable peaks for Hg2+ and S2- were obtained in water based on UV-VIS absorption spectra, the absorbance intensity and/or position of nano-silver vary with the addition of Hg2+ cation and S2- anion, accompanying with color change. Impressively, the optimal AgNPs anchored CMS exhibited a high sensitivity and selectivity toward Hg2+ and S2-, the change in absorbance was linear with the concentration of Hg2+ (0-50 ?M) and S2- (15-70 ?M), and the lowest limits of detection (LOD) were 1.8 × 10-8 M and 2.4 × 10-7 M, respectively. More importantly, owing to the superior properties in testing Hg2+ and S2-, the fabricated sensor was successfully applied for detection of target ions in lake and tap water samples. All these good results implied that the designed strategy and as-designed samples is promising in detecting cation (Hg2+) and anion (S2-) ions and open up new opportunities for selecting other kinds of ions.

SUBMITTER: Xue Y

PROVIDER: S-EPMC7023171 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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A Green, Rapid and Efficient Dual-Sensors for Highly Selective and Sensitive Detection of Cation (Hg2+) and Anion (S2-) Ions Based on CMS/AgNPs Composites.

Xue Yun Y Ma Lina L Zhang Lei L Zhao Wanting W Li Zichao Z Li Qun Q

Polymers 20200105 1

Detection of mercury (Hg2+) and sulfide (S2-), universal and well-known toxic ions, is crucial in monitoring several diseases. How to design and fabricate the high-performance sensor for simultaneously and accurately detecting the Hg2+ and S2- is critical. Herein, we proposed a novel and convenient strategy for optical detection of Hg2+ and S2- by employing a carboxymethyl cellulose sodium/silver nanoparticle (CMS/AgNPs) colloidal ...[more]

PMID: 31948031

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Project description:Cation and anion channelrhodopsins (CCRs and ACRs, respectively) primarily from two algal species, Chlamydomonas reinhardtii and Guillardia theta, have become widely used as optogenetic tools to control cell membrane potential with light. We mined algal and other protist polynucleotide sequencing projects and metagenomic samples to identify 75 channelrhodopsin homologs from four channelrhodopsin families, including one revealed in dinoflagellates in this study. We carried out electrophysiological analysis of 33 natural channelrhodopsin variants from different phylogenetic lineages and 10 metagenomic homologs in search of sequence determinants of ion selectivity, photocurrent desensitization, and spectral tuning in channelrhodopsins. Our results show that association of a reduced number of glutamates near the conductance path with anion selectivity depends on a wider protein context, because prasinophyte homologs with a glutamate pattern identical to that in cryptophyte ACRs are cation selective. Desensitization is also broadly context dependent, as in one branch of stramenopile ACRs and their metagenomic homologs, its extent roughly correlates with phylogenetic relationship of their sequences. Regarding spectral tuning, we identified two prasinophyte CCRs with red-shifted spectra to 585 nm. They exhibit a third residue pattern in their retinal-binding pockets distinctly different from those of the only two types of red-shifted channelrhodopsins known (i.e., the CCR Chrimson and RubyACRs). In cryptophyte ACRs we identified three specific residue positions in the retinal-binding pocket that define the wavelength of their spectral maxima. Lastly, we found that dinoflagellate rhodopsins with a TCP motif in the third transmembrane helix and a metagenomic homolog exhibit channel activity. IMPORTANCE Channelrhodopsins are widely used in neuroscience and cardiology as research tools and are considered prospective therapeutics, but their natural diversity and mechanisms remain poorly characterized. Genomic and metagenomic sequencing projects are producing an ever-increasing wealth of data, whereas biophysical characterization of the encoded proteins lags behind. In this study, we used manual and automated patch clamp recording of representative members of four channelrhodopsin families, including a family in dinoflagellates that we report in this study. Our results contribute to a better understanding of molecular determinants of ionic selectivity, photocurrent desensitization, and spectral tuning in channelrhodopsins.

Dataset Information

A Green, Rapid and Efficient Dual-Sensors for Highly Selective and Sensitive Detection of Cation (Hg2+) and Anion (S2-) Ions Based on CMS/AgNPs Composites.

Publications

A Green, Rapid and Efficient Dual-Sensors for Highly Selective and Sensitive Detection of Cation (Hg<sup>2+</sup>) and Anion (S<sup>2-</sup>) Ions Based on CMS/AgNPs Composites.

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