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Clinical, microbiological, and immunological evaluation of patients in corrective orthodontic treatment.

ABSTRACT: BACKGROUND:The objective was to analyze clinical, microbiological, and immunological periodontal parameters in patients in corrective orthodontic treatment. MATERIALS AND METHODS:Twenty-eight patients were selected. Plaque index (PI), bleeding on probing (BOP), width of keratinized gingiva, levels of 40 bacterial species, and of 3 cytokines (IL-1?, MMP-8, and TNF-?) in gingival crevicular fluid (GCF) were evaluated at T0, before orthodontic treatment; T1, 6?months; and T2, 12?months post-treatment. Non-parametric, Friedman, Wilcoxon, ANOVA, and Spearman correlation coefficient tests were used for statistical analyses, with the significance level of 5%. RESULTS:No significant difference was found for the width of keratinized gingiva, but PI presented a significant increase at T1 and T2 (p??0.05). In the microbiological analysis, red complex pathogens were in significantly greater proportions in T2 compared with T0 (p?

SUBMITTER: Shirozaki MU

PROVIDER: S-EPMC7024686 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Clinical, microbiological, and immunological evaluation of patients in corrective orthodontic treatment.

Shirozaki Mariana Umekita MU da Silva Raquel Assed Bezerra RAB Romano Fábio Lourenço FL da Silva Léa Assed Bezerra LAB De Rossi Andiara A Lucisano Marília Pacífico MP Messora Michel Reis MR Feres Magda M Novaes Júnior Arthur Belém AB

Progress in orthodontics 20200217 1

<h4>Background</h4>The objective was to analyze clinical, microbiological, and immunological periodontal parameters in patients in corrective orthodontic treatment.<h4>Materials and methods</h4>Twenty-eight patients were selected. Plaque index (PI), bleeding on probing (BOP), width of keratinized gingiva, levels of 40 bacterial species, and of 3 cytokines (IL-1β, MMP-8, and TNF-α) in gingival crevicular fluid (GCF) were evaluated at T0, before orthodontic treatment; T1, 6 months; and T2, 12 mont ...[more]

PMID: 32064567

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Project description:BackgroundThe association of scaling and root planing (SRP) with systemic metronidazole (MTZ) plus amoxicillin (AMX) has shown to be an effective treatment protocol, particularly for periodontitis stages III and IV, generalized. More recently, probiotics have also been suggested as a promising adjunctive treatment for periodontal diseases due to their antimicrobial and anti-inflammatory properties. Therefore, the aim of this randomized clinical trial (RCT) is to evaluate the clinical, microbiological, and immunological effects of probiotics as adjuncts to SRP alone or with MTZ+AMX in the treatment of periodontitis.MethodsSubjects with periodontitis are being randomly assigned to receive (i) SRP alone, or with (ii) two probiotic lozenges/day for 90 days (Prob), (iii) MTZ (400 mg) and AMX (500 mg) thrice a day (TID) for 14 days (MTZ+AMX), or (iv) Prob and MTZ+AMX. Subjects are being monitored for up to 12 months post-treatment. Nine subgingival plaque samples per patient are being collected at baseline and at 3, 6, and 12 months post-therapy and analyzed by checkerboard DNA-DNA hybridization for 40 bacterial species. Peripheral blood and gingival crevicular fluid (GCF) of four randomly selected periodontal sites will be analyzed by means of a multiplex fluorescent bead-based immunoassay for 17 cyto/chemokines.Statistical analysesThe significance of differences in each group (over the course of the study) will be sought using repeated measures ANOVA or Friedman tests and among groups (at each time point) using either ANOVA/ANCOVA or Kruskal-Wallis tests, depending on normality of the data. The chi-square test will be used to compare differences in the frequency of subjects achieving the clinical endpoint for treatment (≤ 4 sites with PD ≥ 5 mm) at 1 year and of self-perceived adverse effects. A stepwise forward logistic regression analysis will be performed in order to investigate the impact of different predictor variables on the percentage of patients achieving the clinical endpoint for treatment. The Number Needed to Treat (NNT) with different treatment protocols will be also calculated. Statistical significance will be set at 5%.Trial registrationClinicalTrials.gov NCT03733379. Registered on November 7, 2018.

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Clinical, microbiological, and immunological evaluation of patients in corrective orthodontic treatment.

Publications

Clinical, microbiological, and immunological evaluation of patients in corrective orthodontic treatment.

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