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Mesenchymal stem cells proliferation and remote manipulation upon exposure to magnetic semiconductor nanoparticles.

ABSTRACT: In this paper, we report on spatial redistribution of bone marrow mesenchymal stem cells loaded with magnetic nanoparticles under the influence of continuously applied magnetic field. Semiconductor nanoparticles were synthesized by epitaxial growth of a GaN thin layer on magnetic sacrificial core consisting of ZnFe2O4 nanoparticles. Different quantities of nanoparticles were incubated in vitro with mesenchymal stem cells. High density of nanoparticles (50??g/ml) leads to a decrease in the number of cells during incubation, while the density of nanoparticles as low as 10??g/ml is enough to drag cells in culture and rearrange them according to the spatial distribution of the magnetic field intensity.

SUBMITTER: Braniste T

PROVIDER: S-EPMC7025179 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Mesenchymal stem cells proliferation and remote manipulation upon exposure to magnetic semiconductor nanoparticles.

Braniste Tudor T Cobzac Vitalie V Ababii Polina P Plesco Irina I Raevschi Simion S Didencu Alexandru A Maniuc Mihail M Nacu Viorel V Ababii Ion I Tiginyanu Ion I

Biotechnology reports (Amsterdam, Netherlands) 20200211

In this paper, we report on spatial redistribution of bone marrow mesenchymal stem cells loaded with magnetic nanoparticles under the influence of continuously applied magnetic field. Semiconductor nanoparticles were synthesized by epitaxial growth of a GaN thin layer on magnetic sacrificial core consisting of ZnFe2O4 nanoparticles. Different quantities of nanoparticles were incubated in vitro with mesenchymal stem cells. High density of nanoparticles (50 μg/ml) lead ...[more]

PMID: 32090026

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Project description:Purpose: Iron oxide based magnetic nanoparticles (MNP) are versatile tools in biology and medicine. Adipose derived mesenchymal stem cells (ADSC) and Wharton Jelly mesenchymal stem cells (WJMSC) are currently tested in different strategies for regenerative regenerative medicine (RM) purposes. Their superiority compared to other mesenchymal stem cell consists in larger availability, and superior proliferative and differentiation potential. Magnetic field (MF) exposure of MNP-loaded ADSC has been proposed as a method to deliver mechanical stimulation for increasing conversion to musculoskeletal lineages. In this study, we investigated comparatively chondrogenic conversion of ADSC-MNP and WJMSC with or without MF exposure in order to identify the most appropriate cell source and differentiation protocol for future cartilage engineering strategies. Methods: Human primary ADSC and WJMSC from various donors were loaded with proprietary uncoated MNP. The in vitro effect on proliferation and cellular senescence (beta galactosidase assay) in long term culture was assessed. In vitro chondrogenic differentiation in pellet culture system, with or without MF exposure, was assessed using pellet histology (Safranin O staining) as well as quantitative evaluation of glycosaminoglycan (GAG) deposition per cell. Results: ADSC-MNP complexes displayed superior proliferative capability and decreased senescence after long term (28 days) culture in vitro compared to non-loaded ADSC and to WJMSC-MNP. Significant increase in chondrogenesis conversion in terms of GAG/cell ratio could be observed in ADSC-MNP. MF exposure increased glycosaminoglycan deposition in MNP-loaded ADSC, but not in WJMSC. Conclusion: ADSC-MNP display decreased cellular senescence and superior chondrogenic capability in vitro compared to non-loaded cells as well as to WJMSC-MNP. MF exposure further increases ADSC-MNP chondrogenesis in ADSC, but not in WJMSC. Loading ADSC with MNP can derive a successful procedure for obtaining improved chondrogenesis in ADSC. Further in vivo studies are needed to confirm the utility of ADSC-MNP complexes for cartilage engineering.

Dataset Information

Mesenchymal stem cells proliferation and remote manipulation upon exposure to magnetic semiconductor nanoparticles.

Publications

Mesenchymal stem cells proliferation and remote manipulation upon exposure to magnetic semiconductor nanoparticles.

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