Ontology highlight
ABSTRACT:
SUBMITTER: Arranz-Solis D
PROVIDER: S-EPMC7025762 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
International journal for parasitology 20180403 8
Herein we describe, to our knowledge for the first time the use of the clustered regularly interspaced short palindromic repeats/CRISPR-associated gene 9 (CRISPR/Cas9) system for genome editing of Neospora caninum, an apicomplexan parasite considered one of the main causes of abortion in cattle worldwide. By using plasmids containing the CRISPR/Cas9 components adapted to the closely related parasite Toxoplasma gondii, we successfully knocked out a green fluorescent protein (GFP) in an Nc-1 GFP-e ...[more]