Dataset Information

Quantitative scoring of epithelial and mesenchymal qualities of cancer cells using machine learning and quantitative phase imaging.

ABSTRACT:

Significance

We introduce an application of machine learning trained on optical phase features of epithelial and mesenchymal cells to grade cancer cells' morphologies, relevant to evaluation of cancer phenotype in screening assays and clinical biopsies.

Aim

Our objective was to determine quantitative epithelial and mesenchymal qualities of breast cancer cells through an unbiased, generalizable, and linear score covering the range of observed morphologies.

Approach

Digital holographic microscopy was used to generate phase height maps of noncancerous epithelial (Gie-No3B11) and fibroblast (human gingival) cell lines, as well as MDA-MB-231 and MCF-7 breast cancer cell lines. Several machine learning algorithms were evaluated as binary classifiers of the noncancerous cells that graded the cancer cells by transfer learning.

Results

Epithelial and mesenchymal cells were classified with 96% to 100% accuracy. Breast cancer cells had scores in between the noncancer scores, indicating both epithelial and mesenchymal morphological qualities. The MCF-7 cells skewed toward epithelial scores, while MDA-MB-231 cells skewed toward mesenchymal scores. Linear support vector machines (SVMs) produced the most distinct score distributions for each cell line.

Conclusions

The proposed epithelial-mesenchymal score, derived from linear SVM learning, is a sensitive and quantitative approach for detecting epithelial and mesenchymal characteristics of unknown cells based on well-characterized cell lines. We establish a framework for rapid and accurate morphological evaluation of single cells and subtle phenotypic shifts in imaged cell populations.

SUBMITTER: Lam V

PROVIDER: S-EPMC7026523 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Publications

Quantitative scoring of epithelial and mesenchymal qualities of cancer cells using machine learning and quantitative phase imaging.

Lam Van V Nguyen Thanh T Bui Vy V Chung Byung Min BM Chang Lin-Ching LC Nehmetallah George G Raub Christopher C

Journal of biomedical optics 20200201 2

<h4>Significance</h4>We introduce an application of machine learning trained on optical phase features of epithelial and mesenchymal cells to grade cancer cells' morphologies, relevant to evaluation of cancer phenotype in screening assays and clinical biopsies.<h4>Aim</h4>Our objective was to determine quantitative epithelial and mesenchymal qualities of breast cancer cells through an unbiased, generalizable, and linear score covering the range of observed morphologies.<h4>Approach</h4>Digital h ...[more]

PMID: 32072775

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Project description:Malaria detection through microscopic examination of stained blood smears is a diagnostic challenge that heavily relies on the expertise of trained microscopists. This paper presents an automated analysis method for detection and staging of red blood cells infected by the malaria parasite Plasmodium falciparum at trophozoite or schizont stage. Unlike previous efforts in this area, this study uses quantitative phase images of unstained cells. Erythrocytes are automatically segmented using thresholds of optical phase and refocused to enable quantitative comparison of phase images. Refocused images are analyzed to extract 23 morphological descriptors based on the phase information. While all individual descriptors are highly statistically different between infected and uninfected cells, each descriptor does not enable separation of populations at a level satisfactory for clinical utility. To improve the diagnostic capacity, we applied various machine learning techniques, including linear discriminant classification (LDC), logistic regression (LR), and k-nearest neighbor classification (NNC), to formulate algorithms that combine all of the calculated physical parameters to distinguish cells more effectively. Results show that LDC provides the highest accuracy of up to 99.7% in detecting schizont stage infected cells compared to uninfected RBCs. NNC showed slightly better accuracy (99.5%) than either LDC (99.0%) or LR (99.1%) for discriminating late trophozoites from uninfected RBCs. However, for early trophozoites, LDC produced the best accuracy of 98%. Discrimination of infection stage was less accurate, producing high specificity (99.8%) but only 45.0%-66.8% sensitivity with early trophozoites most often mistaken for late trophozoite or schizont stage and late trophozoite and schizont stage most often confused for each other. Overall, this methodology points to a significant clinical potential of using quantitative phase imaging to detect and stage malaria infection without staining or expert analysis.

Dataset Information

Quantitative scoring of epithelial and mesenchymal qualities of cancer cells using machine learning and quantitative phase imaging.

Significance

Aim

Approach

Results

Conclusions

Publications

Quantitative scoring of epithelial and mesenchymal qualities of cancer cells using machine learning and quantitative phase imaging.

Similar Datasets

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