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Periodic Nucleation of Calcium Phosphate in a Stirred Biocatalytic Reaction.


ABSTRACT: Highly ordered superstructures composed of inorganic nanoparticles appear in natural and synthetic systems, however the mechanisms of non-equilibrium self-organization that may be involved are still poorly understood. Herein, we performed a kinetic investigation of the precipitation of calcium phosphate using a process widely found in microorganisms: the hydrolysis of urea by enzyme urease. With high initial ratio of calcium ion to phosphate, periodic precipitation was obtained accompanied by pH oscillations in a well-stirred, closed reactor. We propose that an internal pH-regulated change in the concentration of phosphate ion is the driving force for periodicity. A simple model involving the biocatalytic reaction network coupled with burst nucleation of nanoparticles above a critical supersaturation reproduced key features of the experiments. These findings may provide insight to the self-organization of nanoparticles in biomineralization and improve design strategies of biomaterials for medical applications.

SUBMITTER: Bohner B 

PROVIDER: S-EPMC7027757 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Periodic Nucleation of Calcium Phosphate in a Stirred Biocatalytic Reaction.

Bohner Bíborka B   Bánsági Tamás T   Tóth Ágota Á   Horváth Dezső D   Taylor Annette F AF  

Angewandte Chemie (International ed. in English) 20200109 7


Highly ordered superstructures composed of inorganic nanoparticles appear in natural and synthetic systems, however the mechanisms of non-equilibrium self-organization that may be involved are still poorly understood. Herein, we performed a kinetic investigation of the precipitation of calcium phosphate using a process widely found in microorganisms: the hydrolysis of urea by enzyme urease. With high initial ratio of calcium ion to phosphate, periodic precipitation was obtained accompanied by pH  ...[more]

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