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Crosstalk between DNA methylation and gene expression in colorectal cancer, a potential plasma biomarker for tracing this tumor.

ABSTRACT: Colorectal cancer (CRC), the second leading cause of cancer mortality, constitutes a significant global health burden. An accurate, noninvasive detection method for CRC as complement to colonoscopy could improve the effectiveness of treatment. In the present study, SureSelectXT Methyl-Seq was performed on cancerous and normal colon tissues and CLDN1, INHBA and SLC30A10 were found as candidate methylated genes. MethyLight assay was run on formalin-fixed paraffin-embedded (FFPE) and fresh case and control tissues to validate the methylation of the selected gene. The methylation was significantly different (p-values?

SUBMITTER: Kerachian MA

PROVIDER: S-EPMC7028731 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Crosstalk between DNA methylation and gene expression in colorectal cancer, a potential plasma biomarker for tracing this tumor.

Kerachian Mohammad Amin MA Javadmanesh Ali A Azghandi Marjan M Mojtabanezhad Shariatpanahi Afsaneh A Yassi Maryam M Shams Davodly Ehsan E Talebi Amin A Khadangi Fatemeh F Soltani Ghodratollah G Hayatbakhsh Abdorasool A Ghaffarzadegan Kamran K

Scientific reports 20200218 1

Colorectal cancer (CRC), the second leading cause of cancer mortality, constitutes a significant global health burden. An accurate, noninvasive detection method for CRC as complement to colonoscopy could improve the effectiveness of treatment. In the present study, SureSelectXT Methyl-Seq was performed on cancerous and normal colon tissues and CLDN1, INHBA and SLC30A10 were found as candidate methylated genes. MethyLight assay was run on formalin-fixed paraffin-embedded (FFPE) and fresh case and ...[more]

PMID: 32071364

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