In vitro replication highlights the mutability of prions.
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ABSTRACT:
SUBMITTER: Vanni I
PROVIDER: S-EPMC7030905 | biostudies-literature | 2014 Jan-Feb
REPOSITORIES: biostudies-literature
Publications
In vitro replication highlights the mutability of prions.
Vanni Ilaria I Di Bari Michele Angelo MA Pirisinu Laura L D'Agostino Claudia C Agrimi Umberto U Nonno Romolo R
Prion 20140101 1
Prions exist as strains, which are thought to reflect PrP(Sc) conformational variants. Prion strains can mutate and it has been proposed that prion mutability depends on an intrinsic heterogeneity of prion populations that would behave as quasispecies. We investigated in vitro prion mutability of 2 strains, by following PrP(Sc) variations of populations serially propagated in PMCA under constant environmental pressure. Each strain was propagated either at low dilution of the seed, i.e., by large ...[more]
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