Ontology highlight
ABSTRACT:
SUBMITTER: Chen CH
PROVIDER: S-EPMC7031464 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Chen Ching-Hsun CH Tseng I-Ting IT Lo Shou-Chen SC Yu Zi-Rong ZR Pang Ju-Jiun JJ Chen Yu-Hsuan YH Huang Chieh-Chen CC Li Si-Yu SY
3 Biotech 20200219 3
The reductive tricarboxylic acid (rTCA) cycle was reconstructed in <i>Escherichia coli</i> by introducing pGETS118KAFS, where <i>kor</i> (encodes α-ketoglutarate:ferredoxin oxidoreductase), <i>acl</i> (encodes ATP-dependent citrate lyase), <i>frd</i> (encodes fumarate reductase), and <i>sdh</i> (encodes succinate dehydrogenase) were tandemly conjugated by the ordered gene assembly in <i>Bacillus subtilis</i> (OGAB). <i>E. coli</i> MZLF (<i>E. coli</i> BL21(DE3) Δ<i>zwf</i>, Δ<i>ldh</i>, Δ<i>frd< ...[more]