Cyclodextrin/chitosan nanoparticles for oral ovalbumin delivery: Preparation, characterization and intestinal mucosal immunity in mice.

ABSTRACT: A novel oral protein delivery system with enhanced intestinal penetration and improved antigen stability based on chitosan (CS) nanoparticles and antigen-cyclodextrin (CD) inclusion complex was prepared by a precipitation/coacervation method. Ovalbumin (OVA) as a model antigen was firstly encapsulated by cyclodextrin, either ?-cyclodextrin (?-CD) or carboxymethyl-hydroxypropyl-?-cyclodextrin (CM-HP-?-CD) and formed OVA-CD inclusion complexes, which were then loaded to chitosan nanoparticles to form OVA loaded ?-CD/CS or CM-HP-?-CD/CS nanoparticles with uniform particle size (836.3 and 779.2?nm, respectively) and improved OVA loading efficiency (27.6% and 20.4%, respectively). In vitro drug release studies mimicking oral delivery condition of OVA loaded CD/CS nanoparticles showed low initial releases at pH 1.2 for 2?h less than 3.0% and a delayed release which was below to 30% at pH 6.8 for further 72?h. More importantly, after oral administration of OVA loaded ?-CD/CS nanoparticles to Balb/c mice, OVA-specific sIgA levels in jejunum of OVA loaded ?-CD/CS nanoparticles were 3.6-fold and 1.9-fold higher than that of OVA solution and OVA loaded chitosan nanoparticles, respectively. In vivo evaluation results showed that OVA loaded CD/CS nanoparticles could enhance its efficacy for inducing intestinal mucosal immune response. In conclusion, our data suggested that CD/CS nanoparticles could serve as a promising antigen-delivery system for oral vaccination.

SUBMITTER: He M 

PROVIDER: S-EPMC7032233 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature