Dataset Information

Correlating the mass and mechanical property changes during the degradation of PEG-based adhesive.

ABSTRACT: Change in mechanical property of a degrading adhesive is critical to its performance. However, characterization of degradation behavior is often limited to tracking its mass loss. 4-armed PEG end modified with dopamine (PEG-DA) was used as a model bioadhesive to correlate its change in mass with change in mechanical property. Shear modulus (G) was calculated based on the mass and average molecular weight between crosslinks (M¯c) of PEG-DA, while the storage modulus (G') was determined by oscillatory rheometry. G decreased slowly within the first week of degradation (10% reduction by week 2), while G' decreased by 60% during the same period. This large discrepancy is due to the partially disconnected and elastically ineffective PEG polymer, which is trapped within the adhesive network. This resulted in minimal mass change and higher calculated G value during the earlier time points. Therefore, tracking mass loss profile alone is inadequate to completely describe the degradation behavior of an adhesive. Additionally, PEG-DA was coated onto magnetoelastic (ME) sensors, and the change in the resonance amplitude of the sensor corresponded well with dry mass loss of PEG-DA. ME sensing provide a non-destructive method to track the mass loss of the coated adhesive.

SUBMITTER: Zhang Z

PROVIDER: S-EPMC7034855 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Correlating the mass and mechanical property changes during the degradation of PEG-based adhesive.

Zhang Zhongtian Z Pinnaratip Rattapol R Ong Keat G KG Lee Bruce P BP

Journal of applied polymer science 20190906 10

Change in mechanical property of a degrading adhesive is critical to its performance. However, characterization of degradation behavior is often limited to tracking its mass loss. 4-armed PEG end modified with dopamine (PEG-DA) was used as a model bioadhesive to correlate its change in mass with change in mechanical property. Shear modulus (<i>G</i>) was calculated based on the mass and average molecular weight between crosslinks ( M ¯ c ) of PEG-DA, while the storage modulus (<i>G'</i>) wa ...[more]

PMID: 32089564

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Project description:Cadherin-based adherens junctions and desmosomes help stabilize cell-cell contacts with additional function in mechano-signaling, while clustered protocadherin junctions are responsible for directing neuronal circuits assembly. Structural models for adherens junctions formed by epithelial cadherin (CDH1) proteins indicate that their long, curved ectodomains arrange to form a periodic, two-dimensional lattice stabilized by tip-to-tip trans interactions (across junction) and lateral cis contacts. Less is known about the exact architecture of desmosomes, but desmoglein (DSG) and desmocollin (DSC) cadherin proteins are also thought to form ordered junctions. In contrast, clustered protocadherin (PCDH)-based cell-cell contacts in neuronal tissues are thought to be responsible for self-recognition and avoidance, and structural models for clustered PCDH junctions show a linear arrangement in which their long and straight ectodomains form antiparallel overlapped trans complexes. Here, we report all-atom molecular dynamics simulations testing the mechanics of minimalistic adhesive junctions formed by CDH1, DSG2 coupled to DSC1, and PCDHγB4, with systems encompassing up to 3.7 million atoms. Simulations generally predict a favored shearing pathway for the adherens junction model and a two-phased elastic response to tensile forces for the adhesive adherens junction and the desmosome models. Complexes within these junctions first unbend at low tensile force and then become stiff to unbind without unfolding. However, cis interactions in both the CDH1 and DSG2-DSC1 systems dictate varied mechanical responses of individual dimers within the junctions. Conversely, the clustered protocadherin PCDHγB4 junction lacks a distinct two-phased elastic response. Instead, applied tensile force strains trans interactions directly, as there is little unbending of monomers within the junction. Transient intermediates, influenced by new cis interactions, are observed after the main rupture event. We suggest that these collective, complex mechanical responses mediated by cis contacts facilitate distinct functions in robust cell-cell adhesion for classical cadherins and in self-avoidance signaling for clustered PCDHs.

Dataset Information

Correlating the mass and mechanical property changes during the degradation of PEG-based adhesive.

Publications

Correlating the mass and mechanical property changes during the degradation of PEG-based adhesive.

Similar Datasets

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