Unknown

Dataset Information

0

Z-Ajoene Inhibits Growth of Colon Cancer by Promotion of CK1α Dependent β-Catenin Phosphorylation.


ABSTRACT: Aberrant activation of a Wnt/β-catenin pathway results in nuclear accumulation of β-catenin in colon cancer. Inhibiting β-catenin is one strategy for treating colon cancer. Here, we identified Z-ajoene, a sulfur containing compound isolated from crushed garlic, as an inhibitor of colon cancer cell growth. Z-Ajoene repressed β-catenin response transcriptional activity, intracellular β-catenin levels, and its representative target protein levels (c-Myc and cyclin D1) in SW480 colon cancer cells. To clarify the regulatory mechanism of decreased β-catenin levels, we examined the effect of Z-ajoene on β-catenin phosphorylation, which is involved in β-catenin degradation. Z-Ajoene promoted the phosphorylation of β-catenin at Ser45 in a casein kinase 1α (CK1α)-dependent manner, which is an essential step in β-catenin degradation in the cytosol. These findings indicate that Z-ajoene from garlic may be a potential chemotherapeutic agent by modulating CK1α activity and the Wnt/β-catenin signaling pathway.

SUBMITTER: Li H 

PROVIDER: S-EPMC7037095 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC4611300 | biostudies-literature
| S-EPMC2043567 | biostudies-literature
| S-EPMC3475258 | biostudies-literature
2011-01-28 | E-GEOD-26850 | biostudies-arrayexpress
| S-EPMC4811767 | biostudies-literature
| S-EPMC2631972 | biostudies-literature
| S-EPMC3033592 | biostudies-literature
| S-EPMC6884968 | biostudies-literature
| S-EPMC4264928 | biostudies-literature
2011-01-28 | GSE26850 | GEO