Ontology highlight
ABSTRACT:
SUBMITTER: Young KL
PROVIDER: S-EPMC7037427 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Young Kenneth L KL Kassouf Claudia C Dolinska Monika B MB Anderson David Eric DE Sergeev Yuri V YV
International journal of molecular sciences 20200130 3
Human tyrosinase (Tyr) is involved in pigment biosynthesis, where mutations in its corresponding gene TYR have been linked to oculocutaneous albinism 1, an autosomal recessive disorder. Although the enzymatic capabilities of Tyr have been well-characterized, the thermodynamic driving forces underlying melanogenesis remain unknown. Here, we analyze protein binding using the diphenol oxidase behavior of Tyr and van 't Hoff temperature-dependent analysis. Recombinant Tyr was expressed and purified ...[more]