DNA targeting by Clostridium cellulolyticum CRISPR-Cas9 Type II-C system.
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ABSTRACT:
SUBMITTER: Fedorova I
PROVIDER: S-EPMC7038990 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Publications
DNA targeting by Clostridium cellulolyticum CRISPR-Cas9 Type II-C system.
Fedorova Iana I Arseniev Anatolii A Selkova Polina P Pobegalov Georgii G Goryanin Ignatiy I Vasileva Aleksandra A Musharova Olga O Abramova Marina M Kazalov Maksim M Zyubko Tatyana T Artamonova Tatyana T Artamonova Daria D Shmakov Sergey S Khodorkovskii Mikhail M Severinov Konstantin K
Nucleic acids research 20200201 4
Type II CRISPR-Cas9 RNA-guided nucleases are widely used for genome engineering. Type II-A SpCas9 protein from Streptococcus pyogenes is the most investigated and highly used enzyme of its class. Nevertheless, it has some drawbacks, including a relatively big size, imperfect specificity and restriction to DNA targets flanked by an NGG PAM sequence. Cas9 orthologs from other bacterial species may provide a rich and largely untapped source of biochemical diversity, which can help to overcome the l ...[more]
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