DMN-Tre Labeling for Detection and High-Content Screening of Compounds against Intracellular Mycobacteria.
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ABSTRACT:
SUBMITTER: Sahile HA
PROVIDER: S-EPMC7045496 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
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DMN-Tre Labeling for Detection and High-Content Screening of Compounds against Intracellular Mycobacteria.
Sahile Henok A HA Rens Celine C Shapira Tirosh T Andersen Raymond J RJ Av-Gay Yossef Y
ACS omega 20200214 7
4-<i>N</i>,<i>N</i>-Dimethylamino-1,8-naphthalimide conjugate of trehalose (DMN-Tre) is a fluorogenic dye recently developed as a diagnostic tool for tuberculosis. DMN-Tre selectively labels the mycobacterial cell wall through the Ag85 enzymes. In this work, we disclose a protocol describing the total synthesis of DMN-Tre with more than 99% purity. We further developed a protocol for in vitro and intercellular labeling of various mycobacterial strains. DMN-Tre labeling was found to be a useful t ...[more]
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