Ontology highlight
ABSTRACT:
SUBMITTER: Miyawaki T
PROVIDER: S-EPMC7046771 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Miyawaki Takeyuki T Morikawa Shota S Susaki Etsuo A EA Nakashima Ai A Takeuchi Haruki H Yamaguchi Shun S Ueda Hiroki R HR Ikegaya Yuji Y
Nature communications 20200227 1
Structural elucidation and molecular scrutiny of cerebral vasculature is crucial for understanding the functions and diseases of the brain. Here, we introduce SeeNet, a method for near-complete three-dimensional visualization of cerebral vascular networks with high signal-to-noise ratios compatible with molecular phenotyping. SeeNet employs perfusion of a multifunctional crosslinker, vascular casting by temperature-controlled polymerization of hybrid hydrogels, and a bile salt-based tissue-clear ...[more]