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Visualization and molecular characterization of whole-brain vascular networks with capillary resolution.


ABSTRACT: Structural elucidation and molecular scrutiny of cerebral vasculature is crucial for understanding the functions and diseases of the brain. Here, we introduce SeeNet, a method for near-complete three-dimensional visualization of cerebral vascular networks with high signal-to-noise ratios compatible with molecular phenotyping. SeeNet employs perfusion of a multifunctional crosslinker, vascular casting by temperature-controlled polymerization of hybrid hydrogels, and a bile salt-based tissue-clearing technique optimized for observation of vascular connectivity. SeeNet is capable of whole-brain visualization of molecularly characterized cerebral vasculatures at the single-microvessel level. Moreover, SeeNet reveals a hitherto unidentified vascular pathway bridging cerebral and hippocampal vessels, thus serving as a potential tool to evaluate the connectivity of cerebral vasculature.

SUBMITTER: Miyawaki T 

PROVIDER: S-EPMC7046771 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Visualization and molecular characterization of whole-brain vascular networks with capillary resolution.

Miyawaki Takeyuki T   Morikawa Shota S   Susaki Etsuo A EA   Nakashima Ai A   Takeuchi Haruki H   Yamaguchi Shun S   Ueda Hiroki R HR   Ikegaya Yuji Y  

Nature communications 20200227 1


Structural elucidation and molecular scrutiny of cerebral vasculature is crucial for understanding the functions and diseases of the brain. Here, we introduce SeeNet, a method for near-complete three-dimensional visualization of cerebral vascular networks with high signal-to-noise ratios compatible with molecular phenotyping. SeeNet employs perfusion of a multifunctional crosslinker, vascular casting by temperature-controlled polymerization of hybrid hydrogels, and a bile salt-based tissue-clear  ...[more]

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