Dataset Information

Heterotrimeric G protein are involved in the regulation of multiple agronomic traits and stress tolerance in rice.

ABSTRACT: BACKGROUND:The heterotrimeric G protein complex, consisting of G?, G?, and G? subunits, are conserved signal transduction mechanism in eukaryotes. Recent molecular researches had demonstrated that G protein signaling participates in the regulation of yield related traits. However, the effects of G protein genes on yield components and stress tolerance are not well characterized. RESULTS:In this study, we generated heterotrimeric G protein mutants in rice using CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) gene-editing technology. The effects of heterotrimeric G proteins on the regulation of yield components and stress tolerance were investigated. The mutants of gs3 and dep1 generated preferable agronomic traits compared to the wild-type, whereas the mutants of rga1 showed an extreme dwarf phenotype, which led to a dramatic decrease in grain production. The mutants showed improved stress tolerance, especially under salinity treatment. We found four putative extra-large G proteins (PXLG)1-4 that also participate in the regulation of yield components and stress tolerance. A yeast two hybrid showed that the RGB1 might interact with PXLG2 but not with PXLG1, PXLG3 or PXLG4. CONCLUSION:These findings will not only improve our understanding of the repertoire of heterotrimeric G proteins in rice but also contribute to the application of heterotrimeric G proteins in rice breeding.

SUBMITTER: Cui Y

PROVIDER: S-EPMC7048073 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Heterotrimeric G protein are involved in the regulation of multiple agronomic traits and stress tolerance in rice.

Cui Yue Y Jiang Nan N Xu Zhengjin Z Xu Quan Q

BMC plant biology 20200228 1

<h4>Background</h4>The heterotrimeric G protein complex, consisting of Gα, Gβ, and Gγ subunits, are conserved signal transduction mechanism in eukaryotes. Recent molecular researches had demonstrated that G protein signaling participates in the regulation of yield related traits. However, the effects of G protein genes on yield components and stress tolerance are not well characterized.<h4>Results</h4>In this study, we generated heterotrimeric G protein mutants in rice using CRISPR/Cas9 (Cluster ...[more]

PMID: 32111163

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Dataset Information

Heterotrimeric G protein are involved in the regulation of multiple agronomic traits and stress tolerance in rice.

Publications

Heterotrimeric G protein are involved in the regulation of multiple agronomic traits and stress tolerance in rice.

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