Profiling the urinary microbiome in men with calcium-based kidney stones.
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ABSTRACT:
SUBMITTER: Xie J
PROVIDER: S-EPMC7049185 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Publications
Profiling the urinary microbiome in men with calcium-based kidney stones.
Xie Jing J Huang Jian-Sheng JS Huang Xiang-Jiang XJ Peng Jun-Ming JM Yu Zhou Z Yuan Ye-Qing YQ Xiao Ke-Feng KF Guo Ji-Nan JN
BMC microbiology 20200228 1
<h4>Background</h4>The dogma that urine is sterile in healthy individuals has been overturned by recent studies applying molecular-based methods. Mounting evidences indicate that dysbiosis of the urinary microbiota is associated with several urological diseases. In this study, we aimed to investigate the urinary microbiome of male patients with calcium-based kidney stones and compare it with those of healthy individuals.<h4>Results</h4>The diversity of the urinary microbiota in kidney stone pati ...[more]
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Project description:Urinary Microbiome From Patients With Kidney Stones Raw Sequence Reads
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Project description: Not available
| S-EPMC8207319 | biostudies-literature
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| S-EPMC3706363 | biostudies-literature
Project description:Background:Urolithiasis is the process of stone formation in the urinary tract. Its etiology is only partly known, and efficient therapeutic approaches are currently lacking. Metabolomics is increasingly used in biomarkers discovery for its ability to identify mediators of relevant (patho)physiological processes. Amino acids may be involved in kidney stone formation. The aim of the present study was to investigate the presence of an amino acid signature in stone former urine through a targeted metabolomic approach. Methods:A panel of 35 amino acids and derivatives was assessed in urines from 15 stone former patients and 12 healthy subjects by UPLC-MS. Partial Least Squares Discriminant Analysis (PLS-DA) was used to define amino acid profiles of cases and controls. Results and Discussion. Our approach led to the definition of a specific amino acid fingerprint in people with kidney stones. A urinary amino acid profile of stone formers was characterized by lower levels of ?-aminobutyric acid, asparagine, ethanolamine, isoleucine, methionine, phenylalanine, serine, tryptophan, and valine. Metabolomic analysis may lend insights into the pathophysiology of urolithiasis and allow tracking this prevalent condition over time.
| S-EPMC7345965 | biostudies-literature
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| S-EPMC4773489 | biostudies-literature