Triploidy in zebrafish larvae: Effects on gene expression, cell size and cell number, growth, development and swimming performance.

ABSTRACT: There is renewed interest in the regulation and consequences of cell size adaptations in studies on understanding the ecophysiology of ectotherms. Here we test if induction of triploidy, which increases cell size in zebrafish (Danio rerio), makes for a good model system to study consequences of cell size. Ideally, diploid and triploid zebrafish should differ in cell size, but should otherwise be comparable in order to be suitable as a model. We induced triploidy by cold shock and compared diploid and triploid zebrafish larvae under standard rearing conditions for differences in genome size, cell size and cell number, development, growth and swimming performance and expression of housekeeping genes and hsp70.1. Triploid zebrafish have larger but fewer cells, and the increase in cell size ma

SUBMITTER: van de Pol ILE 

PROVIDER: S-EPMC7051096 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature