Dataset Information

Genetic variant of SPARC gene and its association with growth traits in Chinese cattle.

ABSTRACT: SPARC is a cysteine-rich acidic secreted protein. It is a non-collagen component of bone, which is widely distributed in humans and animals and plays an important role. SPARC has been found in a variety of human cancers (breast cancer, stomach cancer, ovarian cancer, etc.) and diabetes-related research. Especially the muscle and fat metabolism are closely related. In this study, we used a DNA pool to detect a new SNP site (g.12454T? > ?C). A total of 616 samples of four breeds of Qinchuan cattle (QC, n=176 ), Xianan cattle (XN, n=160 ), Pinan cattle (PN, n=136 ) and Jiaxian cattle (JX, n=144 ) were analyzed and identified with ARMS-PCR. In addition, we correlated SNP with growth traits and showed significant correlation with growth traits such as rump length, hip width, and body length ( p<0.05 ). Moreover, we tested the SPARC gene expression level in different tissues belonging to XN adult cattle ( n=3 ) and found its high expression in muscle tissues (relative to the kidney). Further, we found the SNP is able to increase the SPARC expression level in skeletal muscle ( n=12 ). According to statistical data, this SNP site may be applied to a molecular marker of an early marker-assisted selection for early growth of beef cattle.

SUBMITTER: Zhang D

PROVIDER: S-EPMC7053509 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Genetic variant of <i>SPARC</i> gene and its association with growth traits in Chinese cattle.

Zhang Danyang D Xu Jiawei J Yang Peng P Wen Yifan Y He Hua H Li Jiaxiao J Liang Juntong J Zheng Yining Y Zhang Zijing Z Wang Xianwei X Yu Xiang X Wang Eryao E Lei Chuzhao C Chen Hong H Huang Yongzhen Y

Archives animal breeding 20200130 1

SPARC is a cysteine-rich acidic secreted protein. It is a non-collagen component of bone, which is widely distributed in humans and animals and plays an important role. SPARC has been found in a variety of human cancers (breast cancer, stomach cancer, ovarian cancer, etc.) and diabetes-related research. Especially the muscle and fat metabolism are closely related. In this study, we used a DNA pool to detect a new SNP site (g.12454T  >  C). A total of 616 samples of four breeds of Qinchuan cattle ...[more]

PMID: 32166110

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Project description:Simple Summary Udder conformation traits are economically important for dairy animals in the dairy industry. Milk production loss can be reduced via a better udder structure in dairy cattle. Udder traits are related to milk production and the somatic cell count, which is a sign of mammary infections (mastitis); as such, it is vital to understand the genetic architecture underlying udder traits in Holstein Friesian cattle for genetic development and long-term selection. Through a GWAS on udder structure traits in Chinese Holstein cattle, we identified several significant single-nucleotide polymorphisms (SNPs) and candidate genes associated with udder traits. The results could provide useful information regarding the genetics architecture of udder structure traits, thus improving the genetic information, health, longevity, and production of dairy cattle. Abstract Udder conformation traits are one of the most economic traits in dairy cows, greatly affecting animal health, milk production, and producer profitability in the dairy industry. Genetic analysis of udder structure and scores have been developed in Holstein cattle. In our research, we conducted a genome-wide association study for five udder traits, including anterior udder attachment (AUA), central suspensory ligament (CSL), posterior udder attachment height (PUAH), posterior udder attachment width (PUAW), and udder depth (UD), in which the fixed and random model circulating probability unification (FarmCPU) model was applied for the association analysis. The heritability and the standard errors of these five udder traits ranged from 0.04 ± 0.00 to 0.49 ± 0.03. Phenotype data were measured from 1000 Holstein cows, and the GeneSeek Genomic Profiler (GGP) Bovine 100 K SNP chip was used to analyze genotypic data in Holstein cattle. For GWAS analysis, 984 individual cows and 84,407 single-nucleotide polymorphisms (SNPs) remained after quality control; a total of 18 SNPs were found at the GW significant threshold (p < 5.90 × 10−7). Many candidate genes were identified within 200kb upstream or downstream of the significant SNPs, which include MGST1, MGST2, MTUS1, PRKN, STXBP6, GRID2, E2F8, CDH11, FOXP1, SLF1, TMEM117, SBF2, GC, ADGRB3, and GCLC. Pathway analysis revealed that 58 Gene Ontology (GO) terms and 18 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were enriched with adjusted p values, and these GO terms and the KEGG pathway analysis were associated with biological information, metabolism, hormonal growth, and development processes. These results could give valuable biological information for the genetic architecture of udder conformation traits in dairy Holstein cattle.

Dataset Information

Genetic variant of SPARC gene and its association with growth traits in Chinese cattle.

Publications

Genetic variant of <i>SPARC</i> gene and its association with growth traits in Chinese cattle.

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