Dataset Information

A Functional Polymorphism in the Promoter Region of Interleukin-12B Increases the Risk of Colorectal Cancer.

ABSTRACT: Objective:To investigate whether the polymorphisms of interleukin-12B (IL-12B) were associated with the risk of developing colorectal cancer (CRC). Patients and Methods. Genotypes of rs17860508 and rs3212227 were determined by polymerase chain reaction with a direct sequencing method in 329 CRC patients and 342 matched healthy control subjects. The expression of IL-12B) were associated with the risk of developing colorectal cancer (CRC). Results:Compared with TTAGAG/TTAGAG genotype of rs17860508, the GC/GC and TTAGAG/GC genotypes may significantly increase the risk of CRC (OR?=?1.81, 95% CI?=?1.18-2.78; OR?=?1.46, 95% CI?=?1.01-2.12, respectively). Furthermore, the mRNA levels of IL-12B) were associated with the risk of developing colorectal cancer (CRC). P=0.009) and TTAGAG/TTAGAG (P=0.009) and TTAGAG/TTAGAG (. Conclusion:These data suggested that the rs17860508 GC/GC genotype might upregulate IL-12B expression at the transcriptional level and thus increase the risk of CRC.IL-12B) were associated with the risk of developing colorectal cancer (CRC).

SUBMITTER: Liu Y

PROVIDER: S-EPMC7054766 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

A Functional Polymorphism in the Promoter Region of Interleukin-12B Increases the Risk of Colorectal Cancer.

Liu Yabin Y Li Binghui B Wang Lili L Kong Dexian D

BioMed research international 20200221

<h4>Objective</h4>To investigate whether the polymorphisms of interleukin-12B (<i>IL-12B</i>) were associated with the risk of developing colorectal cancer (CRC). <i>Patients and Methods</i>. Genotypes of rs17860508 and rs3212227 were determined by polymerase chain reaction with a direct sequencing method in 329 CRC patients and 342 matched healthy control subjects. The expression of <i>IL-12B</i>) were associated with the risk of developing colorectal cancer (CRC).<h4>Results</h4>Compared with ...[more]

PMID: 32149085

Similar Datasets

Project description:Background:The long non-coding RNA H19 plays critical roles in cancer occurrence, development, and progression. The present study is for the first time to evaluate the association of genetic variations in the H19 promoter region with advanced colorectal cancer (CRC) susceptibility, environmental factors, and clinical outcomes. Methods:16 single-nucleotide polymorphisms (SNPs) were identified in the H19 gene promoter by DNA sequencing, and 3 SNPs among which including rs4930101, rs11042170, and rs2735970 further expanded samples with 572 advanced CRC patients and 555 healthy controls. Results:We found that harboring SNP [rs4930101 (P?=?0.009), rs2735970 (P?=?0.003), and rs11042170 (P?=?0.003)] or carrying more than one combined risk genotypes significantly increased the risk for CRC [P?<?0.0001, adjusted OR (95% CI) 6.48 (2.97-14.15)]. In the correlation analysis with environmental factors, rs2735970 and gender, combined risk genotypes (>?1 vs.???1) and family history of cancer demonstrated significant interactions. Furthermore, a remarkably worse clinical outcome was found in combined risk genotypes (>?1 vs.???1), especially in CRC patients with body weight???61 kg, smoking, and first-degree family history of cancer (Log-rank test: P?=?0.006, P?=?0.018, and P?=?0.013, respectively). More importantly, the multivariate Cox regression analyses further verified that combined risk genotypes?>?1 showed a prognostic risk factor for CRC patients with body weight???61 kg (P?=?0.002), smoking (P?=?0.008), and family history of cancer (P?=?0.006). In addition, MDR analysis consistently revealed that the combination of selected SNPs and nine known risk factors showed a better prediction prognosis and represented the best model to predict advanced CRC prognosis. Conclusion:3 SNPs of rs4930101, rs11042170, and rs27359703 among 16 identified SNPs of H19 gene remarkably increased CRC risk. Furthermore, the combined risk genotypes had a significant impact on environmental factors and clinical outcomes in the advanced CRC patients with body weight???61 kg, ever-smoking, and first-degree family history of cancer. These data suggest that H19 promoter SNPs, especially these combined SNPs might be more potentially functional biomarkers in the prediction of advanced CRC risk and prognosis.

Dataset Information

A Functional Polymorphism in the Promoter Region of Interleukin-12B Increases the Risk of Colorectal Cancer.

Publications

A Functional Polymorphism in the Promoter Region of Interleukin-12B Increases the Risk of Colorectal Cancer.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets