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Etiologic features of diarrheagenic microbes in stool specimens from patients with acute diarrhea in Thailand.

ABSTRACT: Many microbial species have been recognized as enteropathogens for humans. Here, we predicted the causative agents of acute diarrhea using data from multiplex quantitative PCR (qPCR) assays targeting 19 enteropathogens. For this, a case-control study was conducted at eight hospitals in Thailand. Stool samples and clinical data were collected from 370 hospitalized patients with acute diarrhea and 370 non-diarrheal controls. Multiple enteropathogens were detected in 75.7% and 13.0% of diarrheal stool samples using multiplex qPCR and bacterial culture methods, respectively. Asymptomatic carriers of enteropathogens were found among 87.8% and 45.7% of individuals by qPCR and culture methods, respectively. These results suggested the complexity of identifying causative agents of diarrhea. An analysis using the quantification cut-off values for clinical relevance drastically reduced pathogen-positive stool samples in control subjects from 87.8% to 0.5%, whereas 48.9% of the diarrheal stool samples were positive for any of the 11 pathogens. Among others, rotavirus, norovirus GII, Shigella/EIEC, and Campylobacter were strongly associated with acute diarrhea (P-value?

SUBMITTER: Okada K

PROVIDER: S-EPMC7055299 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Etiologic features of diarrheagenic microbes in stool specimens from patients with acute diarrhea in Thailand.

Okada Kazuhisa K Wongboot Warawan W Kamjumphol Watcharaporn W Suebwongsa Namfon N Wangroongsarb Piyada P Kluabwang Pipat P Chuenchom Nuttagarn N Swaddiwudhipong Witaya W Wongchai Thanee T Manosuthi Weerawat W Assawapatchara Norrathep N Khum-On Patchanee P Udompat Patpong P Thanee Chareeya C Kitsaran Suwatthiya S Jirapong Lakkana L Jaiwong Charoen C Nedsuwan Supalert S Siripipattanamongkol Chotipong C Okada Pilailuk Akkapaiboon PA Chantaroj Siriporn S Komukai Sho S Hamada Shigeyuki S

Scientific reports 20200304 1

Many microbial species have been recognized as enteropathogens for humans. Here, we predicted the causative agents of acute diarrhea using data from multiplex quantitative PCR (qPCR) assays targeting 19 enteropathogens. For this, a case-control study was conducted at eight hospitals in Thailand. Stool samples and clinical data were collected from 370 hospitalized patients with acute diarrhea and 370 non-diarrheal controls. Multiple enteropathogens were detected in 75.7% and 13.0% of diarrheal st ...[more]

PMID: 32132604

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Dataset Information

Etiologic features of diarrheagenic microbes in stool specimens from patients with acute diarrhea in Thailand.

Publications

Etiologic features of diarrheagenic microbes in stool specimens from patients with acute diarrhea in Thailand.

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