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The Malaria Cell Atlas: Single parasite transcriptomes across the complete Plasmodium life cycle.

ABSTRACT: Malaria parasites adopt a remarkable variety of morphological life stages as they transition through multiple mammalian host and mosquito vector environments. We profiled the single-cell transcriptomes of thousands of individual parasites, deriving the first high-resolution transcriptional atlas of the entire Plasmodium berghei life cycle. We then used our atlas to precisely define developmental stages of single cells from three different human malaria parasite species, including parasites isolated directly from infected individuals. The Malaria Cell Atlas provides both a comprehensive view of gene usage in a eukaryotic parasite and an open-access reference dataset for the study of malaria parasites.

SUBMITTER: Howick VM

PROVIDER: S-EPMC7056351 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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The Malaria Cell Atlas: Single parasite transcriptomes across the complete <i>Plasmodium</i> life cycle.

Howick Virginia M VM Russell Andrew J C AJC Andrews Tallulah T Heaton Haynes H Reid Adam J AJ Natarajan Kedar K Butungi Hellen H Metcalf Tom T Verzier Lisa H LH Rayner Julian C JC Berriman Matthew M Herren Jeremy K JK Billker Oliver O Hemberg Martin M Talman Arthur M AM Lawniczak Mara K N MKN

Science (New York, N.Y.) 20190801 6455

Malaria parasites adopt a remarkable variety of morphological life stages as they transition through multiple mammalian host and mosquito vector environments. We profiled the single-cell transcriptomes of thousands of individual parasites, deriving the first high-resolution transcriptional atlas of the entire <i>Plasmodium berghei</i> life cycle. We then used our atlas to precisely define developmental stages of single cells from three different human malaria parasite species, including parasite ...[more]

PMID: 31439762

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