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Patients Allergic to Fish Tolerate Ray Based on the Low Allergenicity of Its Parvalbumin.

ABSTRACT: BACKGROUND:Clinical reactions to bony fish species are common in patients with allergy to fish and are caused by parvalbumins of the ?-lineage. Cartilaginous fish such as rays and sharks contain mainly ?-parvalbumins and their allergenicity is not well understood. OBJECTIVE:To investigate the allergenicity of cartilaginous fish and their ?-parvalbumins in individuals allergic to bony fish. METHODS:Sensitization to cod, salmon, and ray among patients allergic to cod, salmon, or both (n = 18) was explored by prick-to-prick testing. Clinical reactivity to ray was assessed in 11 patients by food challenges or clinical workup. IgE-binding to ?-parvalbumins (cod, carp, salmon, barramundi, tilapia) and ?-parvalbumins (ray, shark) was determined by IgE-ELISA. Basophil activation tests and skin prick tests were performed with ?-parvalbumins from cod, carp, and salmon and ?-parvalbumins from ray and shark. RESULTS:Tolerance of ray was observed in 10 of 11 patients. Prick-to-prick test reactions to ray were markedly lower than to bony fish (median wheal diameter 2 mm with ray vs 11 mm with cod and salmon). IgE to ?-parvalbumins was lower (median, 0.1 kU/L for ray and shark) than to ?-parvalbumins (median, ?1.65 kU/L). Furthermore, ?-parvalbumins demonstrated a significantly reduced basophil activation capacity compared with ?-parvalbumins (eg, ray vs cod, P < .001; n = 18). Skin prick test further demonstrated lower reactivity to ?-parvalbumins compared with ?-parvalbumins. CONCLUSIONS:Most patients allergic to bony fish tolerated ray, a cartilaginous fish, because of low allergenicity of its ?-parvalbumin. A careful clinical workup and in vitro IgE-testing for cartilaginous fish will improve patient management and may introduce an alternative to bony fish into patients' diet.

SUBMITTER: Kalic T

PROVIDER: S-EPMC7060078 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Patients Allergic to Fish Tolerate Ray Based on the Low Allergenicity of Its Parvalbumin.

Kalic Tanja T Morel-Codreanu Francoise F Radauer Christian C Ruethers Thimo T Taki Aya C AC Swoboda Ines I Hilger Christiane C Hoffmann-Sommergruber Karin K Ollert Markus M Hafner Christine C Lopata Andreas L AL Morisset Martine M Breiteneder Heimo H Kuehn Annette A

The journal of allergy and clinical immunology. In practice 20181122 2

<h4>Background</h4>Clinical reactions to bony fish species are common in patients with allergy to fish and are caused by parvalbumins of the β-lineage. Cartilaginous fish such as rays and sharks contain mainly α-parvalbumins and their allergenicity is not well understood.<h4>Objective</h4>To investigate the allergenicity of cartilaginous fish and their α-parvalbumins in individuals allergic to bony fish.<h4>Methods</h4>Sensitization to cod, salmon, and ray among patients allergic to cod, salmon, ...[more]

PMID: 30471362

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Project description:Candida glabrata is a promising microorganism for organic acid production. The present study aimed to investigate the role of C. glabrata Mediator complex subunit 3 (CgMed3p) in protecting C. glabrata under low-pH conditions. To this end, genes CgMED3A and CgMED3B were deleted, resulting in the double-deletion Cgmed3AB? strain. The final biomass and cell viability levels of Cgmed3AB? decreased by 64.5% and 35.8%, respectively, compared to the wild-type strain results at pH 2.0. In addition, lack of CgMed3ABp resulted in selective repression of a subset of genes in the lipid biosynthesis and metabolism pathways. Furthermore, C18:1, lanosterol, zymosterol, fecosterol, and ergosterol were 13.2%, 80.4%, 40.4%, 78.1%, and 70.4% less abundant, respectively, in the Cgmed3AB? strain. In contrast, the concentration of squalene increased by about 44.6-fold. As a result, membrane integrity, rigidity, and H+-ATPase activity in the Cgmed3AB? strain were reduced by 62.7%, 13.0%, and 50.3%, respectively. In contrast, overexpression of CgMED3AB increased the levels of C18:0, C18:1, and ergosterol by 113.2%, 5.9%, and 26.4%, respectively. Moreover, compared to the wild-type results, dry cell weight and pyruvate production increased, irrespective of pH buffering. These results suggest that CgMED3AB regulates membrane composition, which in turn enables cells to tolerate low-pH stress. We propose that regulation of CgMed3ABp may provide a novel strategy for enhancing low-pH tolerance and increasing organic acid production by C. glabrataIMPORTANCE The objective of this study was to investigate the role of Candida glabrata Mediator complex subunit 3 (CgMed3ABp) and its regulation of gene expression at low pH in C. glabrata We found that CgMed3ABp was critical for cellular survival and pyruvate production during low-pH stress. Measures of the levels of plasma membrane fatty acids and sterol composition indicated that CgMed3ABp could play an important role in regulating homeostasis in C. glabrata We propose that controlling membrane lipid composition may enhance the robustness of C. glabrata for the production of organic acids.

Dataset Information

Patients Allergic to Fish Tolerate Ray Based on the Low Allergenicity of Its Parvalbumin.

Publications

Patients Allergic to Fish Tolerate Ray Based on the Low Allergenicity of Its Parvalbumin.

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