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Candidate genes and gene markers for the resistance to porcine pleuropneumonia.

ABSTRACT: Actinobacillus (A.) pleuropneumoniae is one of the most important respiratory pathogens in global pig production. Antimicrobial treatment and vaccination provide only limited protection, but genetic disease resistance is a very promising alternative for sustainable prophylaxis. Previous studies have discovered multiple QTL that may explain up to 30% of phenotypic variance. Based on these findings, the aim of the present study was to use genomic sequencing to identify genetic markers for resistance to pleuropneumonia in a segregating commercial German Landrace line. 163 pigs were infected with A. pleuropneumoniae Serotype 7 through a standardized aerosol infection method. Phenotypes were accurately defined on a clinical, pathological and microbiological basis. The 58 pigs with the most extreme phenotypes were genotyped by sequencing (next-generation sequencing). SNPs were used in a genome-wide association study. The study identified genome-wide associated SNPs on three chromosomes, two of which were chromosomes of QTL which had been mapped in a recent experiment. Each variant explained up to 20% of the total phenotypic variance. Combined, the three variants explained 52.8% of the variance. The SNPs are located in genes involved in the pathomechanism of pleuropneumonia. This study confirms the genetic background for the host's resistance to pleuropneumonia and indicates a potential role of three candidates on SSC2, SSC12 and SSC15. Favorable gene variants are segregating in commercial populations. Further work is needed to verify the results in a controlled study and to identify the functional QTN.

SUBMITTER: Nietfeld F

PROVIDER: S-EPMC7060169 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Candidate genes and gene markers for the resistance to porcine pleuropneumonia.

Nietfeld Florian F Höltig Doris D Willems Hermann H Valentin-Weigand Peter P Wurmser Christine C Waldmann Karl-Heinz KH Fries Ruedi R Reiner Gerald G

Mammalian genome : official journal of the International Mammalian Genome Society 20200120 1-2

Actinobacillus (A.) pleuropneumoniae is one of the most important respiratory pathogens in global pig production. Antimicrobial treatment and vaccination provide only limited protection, but genetic disease resistance is a very promising alternative for sustainable prophylaxis. Previous studies have discovered multiple QTL that may explain up to 30% of phenotypic variance. Based on these findings, the aim of the present study was to use genomic sequencing to identify genetic markers for resistan ...[more]

PMID: 31960078

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Candidate genes and gene markers for the resistance to porcine pleuropneumonia.

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Candidate genes and gene markers for the resistance to porcine pleuropneumonia.

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