Ontology highlight
ABSTRACT:
SUBMITTER: Sanabria H
PROVIDER: S-EPMC7060211 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Nature communications 20200306 1
We use a hybrid fluorescence spectroscopic toolkit to monitor T4 Lysozyme (T4L) in action by unraveling the kinetic and dynamic interplay of the conformational states. In particular, by combining single-molecule and ensemble multiparameter fluorescence detection, EPR spectroscopy, mutagenesis, and FRET-positioning and screening, and other biochemical and biophysical tools, we characterize three short-lived conformational states over the ns-ms timescale. The use of 33 FRET-derived distance sets, ...[more]