Ontology highlight
ABSTRACT:
SUBMITTER: Drexler HL
PROVIDER: S-EPMC7060811 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Drexler Heather L HL Choquet Karine K Churchman L Stirling LS
Molecular cell 20191212 5
Understanding how splicing events are coordinated across numerous introns in metazoan RNA transcripts requires quantitative analyses of transient RNA processing events in living cells. We developed nanopore analysis of co-transcriptional processing (nano-COP), in which nascent RNAs are directly sequenced through nanopores, exposing the dynamics and patterns of RNA splicing without biases introduced by amplification. Long nano-COP reads reveal that, in human and Drosophila cells, splicing occurs ...[more]