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Comparative Analysis of the Different Dyes' Potential to Assess Human Normal and Cancer Cell Viability In Vitro under Different D/H Ratios in a Culture Medium.

ABSTRACT: In this study, using new approach (laser diffraction?+?biological dyes), we have demonstrated the decrease of cells viability in vitro in the deuterated growth medium, whereas in the deuterium-depleted medium, there was an increase of cell viability. We have also found that not all dyes are equally sensitive to the D/H ratios in the culture medium (system) as well as to the different cell types (cancer vs normal cells).

SUBMITTER: Zlatskiy IA

PROVIDER: S-EPMC7060866 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Comparative Analysis of the Different Dyes' Potential to Assess Human Normal and Cancer Cell Viability In Vitro under Different D/H Ratios in a Culture Medium.

Zlatskiy I A IA Zlatska A V AV Antipova N V NV Dolenko S A SA Gordiienko I M IM Gubar O S OS Vasyliev R G RG Zubov D A DA Novikova S N SN Syroeshkin A V AV

TheScientificWorldJournal 20200225

In this study, using new approach (laser diffraction + biological dyes), we have demonstrated the decrease of cells viability in vitro in the deuterated growth medium, whereas in the deuterium-depleted medium, there was an increase of cell viability. We have also found that not all dyes are equally sensitive to the D/H ratios in the culture medium (system) as well as to the different cell types (cancer vs normal cells). ...[more]

PMID: 32158363

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