Unknown

Dataset Information

0

The Rheb-TORC1 signaling axis functions as a developmental checkpoint.


ABSTRACT: In many eukaryotes, the small GTPase Rheb functions as a switch to toggle activity of TOR complex 1 (TORC1) between anabolism and catabolism, thus controlling lifespan, development and autophagy. Our CRISPR-generated, fluorescently tagged endogenous Caenorhabditis elegans RHEB-1 and DAF-15/Raptor are expressed ubiquitously and localize to lysosomes. LET-363/TOR and DAF-15/Raptor are required for development beyond the third larval stage (L3). We observed that deletion of RHEB-1 similarly conferred L3 arrest. Unexpectedly, robust RNAi-mediated depletion of TORC1 components caused arrest at stages prior to L3. Accordingly, conditional depletion of endogenous DAF-15/Raptor in the soma revealed that TORC1 is required at each stage of the life cycle to progress to the next stage. Reversal of DAF-15 depletion permits arrested animals to recover to continue development. Our results are consistent with TORC1 functioning as a developmental checkpoint that governs the decision of the animal to progress through development.

SUBMITTER: Duong T 

PROVIDER: S-EPMC7063671 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

The Rheb-TORC1 signaling axis functions as a developmental checkpoint.

Duong Tam T   Rasmussen Neal R NR   Ballato Elliot E   Mote F Sefakor FS   Reiner David J DJ  

Development (Cambridge, England) 20200302 5


In many eukaryotes, the small GTPase Rheb functions as a switch to toggle activity of TOR complex 1 (TORC1) between anabolism and catabolism, thus controlling lifespan, development and autophagy. Our CRISPR-generated, fluorescently tagged endogenous <i>Caenorhabditis elegans</i> RHEB-1 and DAF-15/Raptor are expressed ubiquitously and localize to lysosomes. LET-363/TOR and DAF-15/Raptor are required for development beyond the third larval stage (L3). We observed that deletion of RHEB-1 similarly  ...[more]

Similar Datasets

| S-EPMC4764560 | biostudies-literature
| S-EPMC1458976 | biostudies-literature
| S-EPMC9704768 | biostudies-literature
| S-EPMC2812631 | biostudies-literature
| S-EPMC8982600 | biostudies-literature
| S-EPMC4331286 | biostudies-literature
| S-EPMC2962498 | biostudies-literature
| S-EPMC3699820 | biostudies-other
| S-EPMC5803267 | biostudies-literature
| S-EPMC7453609 | biostudies-literature