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The extracellular thioredoxin Etrx3 is required for macrophage infection in Rhodococcus equi.

ABSTRACT: Rhodococcus equi is an intracellular veterinary pathogen that is becoming resistant to current antibiotherapy. Genes involved in preserving redox homeostasis could be promising targets for the development of novel anti-infectives. Here, we studied the role of an extracellular thioredoxin (Etrx3/REQ_13520) in the resistance to phagocytosis. An etrx3-null mutant strain was unable to survive within macrophages, whereas the complementation with the etrx3 gene restored its intracellular survival rate. In addition, the deletion of etrx3 conferred to R. equi a high susceptibility to sodium hypochlorite. Our results suggest that Etrx3 is essential for the resistance of R. equi to specific oxidative agents.

SUBMITTER: Mourenza A

PROVIDER: S-EPMC7063783 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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The extracellular thioredoxin Etrx3 is required for macrophage infection in Rhodococcus equi.

Mourenza Álvaro Á Collado Cristina C Bravo-Santano Natalia N Gil José A JA Mateos Luís M LM Letek Michal M

Veterinary research 20200310 1

Rhodococcus equi is an intracellular veterinary pathogen that is becoming resistant to current antibiotherapy. Genes involved in preserving redox homeostasis could be promising targets for the development of novel anti-infectives. Here, we studied the role of an extracellular thioredoxin (Etrx3/REQ_13520) in the resistance to phagocytosis. An etrx3-null mutant strain was unable to survive within macrophages, whereas the complementation with the etrx3 gene restored its intracellular survival rate ...[more]

PMID: 32156317

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