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A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin.

ABSTRACT: Although phototherapy (PT) is a standard treatment for neonatal jaundice, no validated clinical methods for determination of bilirubin phototherapy products are available. Thus, the aim of our study was to establish a such method for clinical use. To achieve this aim, a LC-MS/MS assay for simultaneous determination of Z-lumirubin (LR) and unconjugated bilirubin (UCB) was conducted. LR was purified after irradiation of UCB at 460?nm. The assay was tested on human sera from PT-treated neonates. Samples were separated on a HPLC system with a triple quadrupole mass spectrometer detector. The instrument response was linear up to 5.8 and 23.4?mg/dL for LR and UCB, respectively, with submicromolar limits of detection and validity parameters relevant for use in clinical medicine. Exposure of newborns to PT raised serum LR concentrations three-fold (p?

SUBMITTER: Jasprova J

PROVIDER: S-EPMC7064611 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin.

Jašprová Jana J Dvořák Aleš A Vecka Marek M Leníček Martin M Lacina Ondřej O Valášková Petra P Zapadlo Miloš M Plavka Richard R Klán Petr P Vítek Libor L

Scientific reports 20200310 1

Although phototherapy (PT) is a standard treatment for neonatal jaundice, no validated clinical methods for determination of bilirubin phototherapy products are available. Thus, the aim of our study was to establish a such method for clinical use. To achieve this aim, a LC-MS/MS assay for simultaneous determination of Z-lumirubin (LR) and unconjugated bilirubin (UCB) was conducted. LR was purified after irradiation of UCB at 460 nm. The assay was tested on human sera from PT-treated neonates. Sa ...[more]

PMID: 32157102

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A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin.

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A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin.

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