Dataset Information

Response of Fc?RI-bearing leucocytes to omalizumab in chronic spontaneous urticaria.

ABSTRACT:

Background

The pathogenesis of chronic spontaneous urticaria (CSU) and the mechanism of action of omalizumab in CSU remain unclear.

Objective

In this study, we assessed the responsiveness and Fc?RI expression of various subsets of leucocytes in patients with CSU treated with omalizumab.

Methods

In this prospective cohort study, 30 patients were treated with 6 administrations of 300 mg omalizumab every 4 weeks, followed by a follow-up period of 12 weeks. Fc?RI expression and the percentage of basophils, monocytes, and dendritic cell subsets were analysed before and during treatment, and after follow-up. In addition, anti-IgE- and C5a-induced basophil degranulation was measured. The results were correlated with disease activity and response to omalizumab.

Results

In addition to a rapid and significant reduction in Fc?RI on basophils, we demonstrated a reduction in Fc?RI on plasmacytoid dendritic cells during omalizumab treatment, which persisted until 3 months after discontinuation. Fc?RI expression on basophils and its reduction did not correlate with the treatment response. Omalizumab led to an increased percentage of basophils in blood but not of the other Fc?RI-bearing leucocytes. Basophil responsiveness was differentially affected; anti-IgE-, but not C5a-induced basophil degranulation increased during the treatment. Apart from clinical non-responders showing a stronger increase in anti-IgE-induced basophil degranulation over a period time, no differences were found in omalizumab responders vs non-responders.

Conclusions/clinical relevance

Fc?RI expression on basophils decreased rapidly, while anti-IgE-induced degranulation significantly increased due to omalizumab treatment in patients with CSU, persisting at least for 3 months after stopping the treatment. None of the markers were able to predict the effectiveness of treatment. Whether basophils play a role in omalizumab responsiveness in CSU remains unclear.

SUBMITTER: Alizadeh Aghdam M

PROVIDER: S-EPMC7065003 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Response of FcεRI-bearing leucocytes to omalizumab in chronic spontaneous urticaria.

Alizadeh Aghdam Mehran M Knol Edward F EF van den Elzen Mignon M den Hartog Jager Constance C van Os-Medendorp Harmieke H Knulst André C AC Otten Henny G HG Röckmann Heike H

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 20200207 3

<h4>Background</h4>The pathogenesis of chronic spontaneous urticaria (CSU) and the mechanism of action of omalizumab in CSU remain unclear.<h4>Objective</h4>In this study, we assessed the responsiveness and FcεRI expression of various subsets of leucocytes in patients with CSU treated with omalizumab.<h4>Methods</h4>In this prospective cohort study, 30 patients were treated with 6 administrations of 300 mg omalizumab every 4 weeks, followed by a follow-up period of 12 weeks. FcεRI expression and ...[more]

PMID: 31925825

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