Dataset Information

Natal origin of the invasive biosecurity pest, brown marmorated stink bug (Halyomorpha halys: Penatomidae), determined by dual-element stable isotope-ratio mass spectrometry.

ABSTRACT: BACKGROUND:Post-border detection of a single brown marmorated stink bug (BMSB) in New Zealand warranted a biosecurity response, the nature of which would be influenced by its status as part of an established population or as a new arrival. Stable isotope analysis has the potential to determine natal origins, but is difficult to achieve for samples as small as a single insect. Here an analytical modification to measure small samples was successfully trialled as a means to supply evidence as to the local or exotic natal origin of the intercepted BMSB specimen. RESULTS:Sufficient analytical sensitivity was achieved using a modified isotope ratio mass spectrometry method, involving thermolysis and carbon monoxide cryofocusing, to enable the simultaneous analysis of ?2 H and ?18 O from wings of the post-border BMSB sample. The values were much lower than those of the New Zealand green vegetable bug, used as a local reference. However, they fell within the range of those for BMSB of Northern Hemisphere origin intercepted at the New Zealand border over the same time period, specifically overlapping with the USA and Italy, but not China. CONCLUSION:The isotope signature of the post-border detected BMSB suggested a significantly cooler climate than the North Island of New Zealand, indicating that it was a new arrival and did not represent an established population. © 2019 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

SUBMITTER: Holder PW

PROVIDER: S-EPMC7065004 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Natal origin of the invasive biosecurity pest, brown marmorated stink bug (Halyomorpha halys: Penatomidae), determined by dual-element stable isotope-ratio mass spectrometry.

Holder Peter W PW Van Hale Robert J RJ Frew Russell R George Sherly S Armstrong Karen F KF

Pest management science 20191209 4

<h4>Background</h4>Post-border detection of a single brown marmorated stink bug (BMSB) in New Zealand warranted a biosecurity response, the nature of which would be influenced by its status as part of an established population or as a new arrival. Stable isotope analysis has the potential to determine natal origins, but is difficult to achieve for samples as small as a single insect. Here an analytical modification to measure small samples was successfully trialled as a means to supply evidence ...[more]

PMID: 31659828

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Project description:Halyomorpha halys (Stål) (Insecta:Hemiptera;Pentatomidae), commonly known as the Brown Marmorated Stink Bug (BMSB), is an invasive pest of the mid-Atlantic region of the United States, causing economically important damage to a wide range of crops. Native to Asia, BMSB was first observed in Allentown, PA, USA, in 1996, and this pest is now well-established throughout the US mid-Atlantic region and beyond. In addition to the serious threat BMSB poses to agriculture, BMSB has become a nuisance to homeowners, invading home gardens and congregating in large numbers in human-made structures, including homes, to overwinter. Despite its significance as an agricultural pest with limited control options, only 100 bp of BMSB sequence data was available in public databases when this project began.Transcriptome sequencing was undertaken to provide a molecular resource to the research community to inform the development of pest control strategies and to provide molecular data for population genetics studies of BMSB. Using normalized, strand-specific libraries, we sequenced pools of all BMSB life stages on the Illumina HiSeq. Trinity was used to assemble 200,000 putative transcripts in >100,000 components. A novel bioinformatic method that analyzed the strand-specificity of the data reduced this to 53,071 putative transcripts from 18,573 components. By integrating multiple other data types, we narrowed this further to 13,211 representative transcripts.Bacterial endosymbiont genes were identified in this dataset, some of which have a copy number consistent with being lateral gene transfers between endosymbiont genomes and Hemiptera, including ankyrin-repeat related proteins, lysozyme, and mannanase. Such genes and endosymbionts may provide novel targets for BMSB-specific biocontrol. This study demonstrates the utility of strand-specific sequencing in generating shotgun transcriptomes and that rapid sequencing shotgun transcriptomes is possible without the need for extensive inbreeding to generate homozygous lines. Such sequencing can provide a rapid response to pest invasions similar to that already described for disease epidemiology.

Project description:Halyomorpha halys (Stål) (Heteroptera: Pentatomidae), the brown marmorated stink bug, is an invasive agricultural and nuisance pest rapidly expanding its incidence in North America. This voracious pest poses a significant threat to rural and urban agriculture, especially to specialty crops such as apples, grapes and ornamentals, as well as staple crops including soybean and corn. The object of this study was to generate transcript sequence resources for H. halys. RNA-seq libraries derived from distinct developmental stages and sexes were sequenced and assembled into 248,569 putatively unique transcripts (PUTs). PUTs were segmented into three disjoint tiers of varying reliability, with 4,794 classified as gold tier (highest quality), 16,878 as silver, and 14,357 as bronze. The gold-tier PUTs associated with 2,580 distinct non-redundant protein sequences from the NCBI NR database--1,785 of these (69%) mapped to annotated UniProtKB database proteins, from which 1,273 unique Pfam families and 459 unique Molecular Function GO terms were encountered. Of the silver tier's 6,527 PUTs associated with unique proteins, 4,193 mapped to UniProtKB (64%), from which 1,941 and 640 unique Pfam and Molecular Function GO terms were extracted. H. halys PUTs related to important life processes like immunity, endocrinology, reproduction, development, behavior, neurotransmission, neurotoxicity, olfaction, and small RNA pathways were validated through quantitative Real-Time PCR (qRT-PCR) for differential expression during distinct life stages (eggs, 2nd instar nymphs, 4th instar nymphs, female adults, male adults). PUTs similar to hypothetical proteins identified in symbiont microbes, including Pantoea and Nosema species, were more abundantly expressed in adults versus nymphs. These comprehensive H. halys transcriptomic resources can be utilized to aid development of novel control methodologies to disrupt life processes; to conduct reverse genetic screens to determine host gene function; and to design environmentally unobtrusive means to control host populations or target specific H. halys life stages, such as molecular biopesticides.

Project description:BackgroundBrown marmorated stink bug (BMSB), Halyomorpha halys (Hemiptera: Pentatomidae) is native to East Asia but has invaded many countries in the world. BMSB is a polyphagous insect pest and causes significant economic losses to agriculture worldwide. Knowledge on the genetic diversity among BMSB populations is scarce but is essential to understand the patterns of colonization and invasion history of local populations. Efforts have been made to assess the genetic diversity of BMSB using partial mitochondrial DNA sequences but genetic divergence on mitochondria is not high enough to precisely accurately identify and distinguish various BMSB populations. Therefore, in this study, we applied a ddRAD (double digest restriction-site associated DNA) sequencing approach to ascertain the genetic diversity of BMSB populations collected from 12 countries (2 native and 10 invaded) across four continents with the ultimate aim to trace the origin of BMSBs intercepted during border inspections and post-border surveillance.ResultA total of 1775 high confidence single nucleotide polymorphisms (SNPs) were identified from ddRAD sequencing data collected from 389 adult BMSB individuals. Principal component analysis (PCA) of the identified SNPs indicated the existence of two main distinct genetic clusters representing individuals sampled from regions where BMSB is native to, China and Japan, respectively, and one broad cluster comprised individuals sampled from countries which have been invaded by BMSB. The population genetic structure analysis further discriminated the genetic diversity among the BMSB populations at a higher resolution and distinguished them into five potential genetic clusters.ConclusionThe study revealed hidden genetic diversity among the studied BMSB populations across the continents. The BMSB populations from Japan were genetically distant from the other studied populations. Similarly, the BMSB populations from China were also genetically differentiated from the Japanese and other populations. Further genetic structure analysis revealed the presence of at least three genetic clusters of BMSB in the invaded countries, possibly originating via multiple invasions. Furthermore, this study has produced novel set of SNP markers to enhance the knowledge of genetic diversity among BMSB populations and demonstrates the potential to trace the origin of BMSB individuals for future invasion events.

Dataset Information

Natal origin of the invasive biosecurity pest, brown marmorated stink bug (Halyomorpha halys: Penatomidae), determined by dual-element stable isotope-ratio mass spectrometry.

Publications

Natal origin of the invasive biosecurity pest, brown marmorated stink bug (Halyomorpha halys: Penatomidae), determined by dual-element stable isotope-ratio mass spectrometry.

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