Ontology highlight
ABSTRACT:
SUBMITTER: Tang X
PROVIDER: S-EPMC7065449 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Tang Xinming X Suo Jingxia J Liang Lin L Duan Chunhui C Hu Dandan D Gu Xiaolong X Yu Yonglan Y Liu Xianyong X Cui Shangjin S Suo Xun X
Veterinary research 20200311 1
Eimeria tenella has emerged as valuable model organism for studying the biology and immunology of protozoan parasites with the establishment of the reverse genetic manipulation platform. In this report, we described the application of CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (endonuclease) system for efficient genetic editing in E. tenella, and showed that the CRISPR/Cas9 system mediates site-specific double-strand DNA breaks with a single guide RNA. Using this syst ...[more]