Ontology highlight
ABSTRACT:
SUBMITTER: Sakamoto S
PROVIDER: S-EPMC7065886 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Science advances 20200311 11
We established an ultrasensitive method for identifying multiple enzymes in biological samples by using a multiplexed microdevice-based single-molecule enzymatic assay. We used a paradigm in which we "count" the number of enzyme molecules by profiling their single enzyme activity characteristics toward multiple substrates. In this proof-of-concept study of the single enzyme activity-based protein profiling (SEAP), we were able to detect the activities of various phosphoric ester-hydrolyzing enzy ...[more]